IHC (Immunohistochemistry)
Mouse Lamin A+C Monoclonal Antibody
Mouse Anti-Lamin A+C [JOL2]
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation
Purity
Unpurified
Synonyms
Lamin A+C, Antibody; Mouse Anti-Lamin A+C [JOL2]; 70 kDa Lamin antibody; Cardiomyopathy dilated 1A (autosomal dominant) antibody; CDCD1 antibody; CDDCantibody; Charcot Marie Tooth Disease Axonal Type 2B1 antibody; CMD1A antibody; CMT2B1 antibody; EMD2antibody; FPL antibody; FPLD antibody; HGPS antibody; IDC antibody; Lamin A/C antibody; Lamin A/C Isoform 1Precursor antibody; Lamin A/C Isoform 2 antibody; Lamin A/C Isoform 3 antibody; LDP1 antibody; LFPantibody; LGMD1B antibody; Limb girdle muscular dystrophy 1B (autosomal dominant) antibody; LMN 1 antibody; LMN Aantibody; LMN C antibody; LMN1 antibody; LMNA antibody; LMNC antibody; NY REN 32 antigen antibody; PRO1antibody; Progeria 1 (Hutchinson Gilford Type) antibody; Renal carcinoma antigen NY REN 32 antibody; Renalcarcinoma antigen NYREN32 antibody; anti-Lamin A+C antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JOL2
Specificity
This antibody reacts with both recombinant and native Lamin A and C in humans. Binds to an epitope between amino acids 464-572
Purity/Purification
Unpurified
Form/Format
Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum, plus 0.09% sodium azide).
Applicable Applications for anti-Lamin A+C antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IP (Immunoprecipitation)
Immunogen
Recombinant human lamin A
Myeloma/fusion Partners
Spleen cells from immunised BALB/C mice were fused with cells of the mouse). SP2-0/Ag4 myeloma cell line.
Health & Safety
Products that contain sodium azide ( a poisonous and hazardous substance) should be handled by trained staff only.
Preparation and Storage
Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
References
Both emerin and lamin C depend on lamin A for localization at the nuclear envelope. Vaughan A (J Cell Sci,Jul 2001)PUBMED Iglesias-Guimarais V et al. Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on a Cytosolic Pool of DFF40/CAD Endonuclease. J Biol Chem 287:7766-79 (2012). WB ; HumanPUBMED Harrigan JA et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol 193:97-108 (2011). ICC/IF ; HumanPUBMED Gudise S et al. Samp1 is functionally associated with the LINC complex and A-type lamina networks. J Cell Sci 124:2077-85 (2011)PUBMED Harten IA et al. Age-Dependent Loss of MMP-3 in Hutchinson-Gilford Progeria Syndrome. J Gerontol A Biol Sci Med Sci : (2011)PUBMED Toulany M et al. Impact of oncogenic K-RAS on YB-1 phosphorylation induced by ionizing radiation. Breast Cancer Res 13:R28 (2011). WB ; HumanPUBMED Mamchaoui K et al. Immortalized pathological human myoblasts: towards a universal tool for the study of neuromuscular disorders. Skelet Muscle 1:34 (2011). ICC/IF ; HumanPUBMED Negroni E et al. In vivo myogenic potential of human CD133+ muscle-derived stem cells: a quantitative study. Mol Ther 17:1771-8 (2009). ICC/IF ; HumanPUBMED
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Product Notes
The Lamin A+C (Catalog #AAA59512) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lamin A+C can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Lamin A+C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin A+C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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