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product-image-AAA30799_APP6.png Application Data

Mouse Lamin B1 Monoclonal Antibody | anti-LMNB1 antibody

Lamin B1 Monoclonal Antibody (7C11)

Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Lamin B1, Antibody; Lamin B1 Monoclonal Antibody (7C11); Lamin-B1; LMNB1; LMN2; LMNB; anti-LMNB1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Clone Number
7C11
Specificity
The antibody detects endogenous Lamin B1 protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-LMNB1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 1:2000-5000
IP: 1:200
IF: 1:200
IHC: 1:50-300
Immunogen
Recombinant Protein of Lamin-B1
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

Application Data

product-image-AAA30799_APP6.png Application Data

Application Data

product-image-AAA30799_APP5.png Application Data

Application Data

(Zhang. Jie. et al. "Neuroprotective effects of astaxanthin against oxygen and glucose deprivation damage via the PI3K/Akt/GSK3?/Nrf2 signalling pathway in vitro." Journal of Cellular and Molecular Medicine 24.16 (2020): 8977-8985.)

product-image-AAA30799_APP4.png Application Data (Zhang. Jie. et al. "Neuroprotective effects of astaxanthin against oxygen and glucose deprivation damage via the PI3K/Akt/GSK3?/Nrf2 signalling pathway in vitro." Journal of Cellular and Molecular Medicine 24.16 (2020): 8977-8985.)

Application Data

(Cai. Shao-Ai. et al. "Nrf2 is a key regulator on puerarin preventing cardiac fibrosis and upregulating metabolic enzymes UGT1A1 in rats." Frontiers in pharmacology 9 (2018).)

product-image-AAA30799_APP3.png Application Data (Cai. Shao-Ai. et al. "Nrf2 is a key regulator on puerarin preventing cardiac fibrosis and upregulating metabolic enzymes UGT1A1 in rats." Frontiers in pharmacology 9 (2018).)

WB (Western Blot)

(Western blot analysis of lysates from 1) HepG2. 2) 293T. 3) Mouse Brain Tissue. 4)Rat Brain Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

product-image-AAA30799_WB2.png WB (Western Blot) (Western blot analysis of lysates from 1) HepG2. 2) 293T. 3) Mouse Brain Tissue. 4)Rat Brain Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot)

(Western blot analysis of 1) HepG2. 2) 293T. 3) Mouse Brain Tissue. 4) Rat Brain Tissue. diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)

product-image-AAA30799_WB.png WB (Western Blot) (Western blot analysis of 1) HepG2. 2) 293T. 3) Mouse Brain Tissue. 4) Rat Brain Tissue. diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
Related Product Information for anti-LMNB1 antibody
lamin B1(LMNB1) Homo sapiens This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Product Categories/Family for anti-LMNB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
586
NCBI Official Full Name
Lamin-B1
NCBI Official Synonym Full Names
lamin B1
NCBI Official Symbol
LMNB1
NCBI Official Synonym Symbols
LMN; ADLD; LMN2; LMNB
NCBI Protein Information
lamin-B1
UniProt Protein Name
Lamin-B1
UniProt Gene Name
LMNB1
UniProt Synonym Gene Names
LMN2; LMNB
UniProt Entry Name
LMNB1_HUMAN

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Product Notes

The LMNB1 lmnb1 (Catalog #AAA30799) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Lamin B1 Monoclonal Antibody (7C11) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:2000-5000 IP: 1:200 IF: 1:200 IHC: 1:50-300. Researchers should empirically determine the suitability of the LMNB1 lmnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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