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product-image-AAA23908_APP11.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Mouse Laminin Receptor/RPSA Monoclonal Antibody | anti-RPSA antibody

Laminin Receptor/RPSA (Marker of Metastatic Potential)

Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
Laminin Receptor/RPSA, Antibody; Laminin Receptor/RPSA (Marker of Metastatic Potential); 34/67kDa Laminin Receptor; 37kDa Laminin Receptor precursor; 37/67kDa Laminin Receptor; 37LRP; 40S Ribosomal Protein SA; 67kDa Laminin Receptor; 67LR; Colon Carcinoma Laminin-binding Protein; LAMBR; Laminin receptor 1; Laminin-binding Protein precursor p40; LAMR1; LBP/p40; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; Ribosomal Protein SA (RPSA); anti-RPSA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
RPSA/2699
Specificity
Laminins, a family of extracellular matrix glycoproteins, are the major non-collagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. Reportedly, level of laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype.
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. (Also available WITHOUT BSA & azide at 1mg/ml.)
Applicable Applications for anti-RPSA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF) Western Blot (WB), Immuohistochemistry (IHC)
Application Notes
Flow Cytometry (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.
Immunogen
Recombinant full-length human RPSA protein
Cellular Localization
Cell Surface, Cytoplasmic and Nuclear
Positive Control
MCF-7, MDA-MB-453, SK-BR3, THP1, HL60, HeLa or HEK293 cells. Breast, Colon or Ovarian Carcinoma, Tonsil.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

product-image-AAA23908_APP11.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IF (Immunofluorescence)

(Immunofluorescent staining of paraformaldehyde-Raji cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

product-image-AAA23908_IF10.jpg IF (Immunofluorescence) (Immunofluorescent staining of paraformaldehyde-Raji cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).)

product-image-AAA23908_FCM9.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699). Confirmation of Integrity and Purity of Antibody)

product-image-AAA23908_SDS_PAGE8.jpg SDS-PAGE (SDS-PAGE Analysis Purified Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699). Confirmation of Integrity and Purity of Antibody)

WB (Western Blot)

(Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_WB7.jpg WB (Western Blot) (Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IF (Immunofluorescence)

(Immunofluorescent staining of HeLa cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

product-image-AAA23908_IF6.jpg IF (Immunofluorescence) (Immunofluorescent staining of HeLa cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_IHC5.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_IHC4.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_IHC3.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_IHC2.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

product-image-AAA23908_IHC.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)
References
W. Al-Saleh, et al.; J. Pathol. 181, 287 (1997).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37-67kDa
NCBI Official Full Name
40S ribosomal protein SA isoform 2
NCBI Official Synonym Full Names
ribosomal protein SA
NCBI Official Symbol
RPSA
NCBI Official Synonym Symbols
SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
NCBI Protein Information
40S ribosomal protein SA
UniProt Protein Name
40S ribosomal protein SA
UniProt Gene Name
RPSA
UniProt Synonym Gene Names
LAMBR; LAMR1; 37LRP

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Product Notes

The RPSA rpsa (Catalog #AAA23908) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Laminin Receptor/RPSA (Marker of Metastatic Potential) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin Receptor/RPSA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF) Western Blot (WB), Immuohistochemistry (IHC). Flow Cytometry (1-2ug/ml) Immunofluorescence (1-2ug/ml) Western Blot (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the RPSA rpsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin Receptor/RPSA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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