WB (Western Blot)
(Detection of human and mouse RPSA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPSA antibody AAA213202 (lot AAA213202-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RPSA Polyclonal Antibody | anti-RPSA antibody
Rabbit anti-RPSA Antibody, Affinity Purified
Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RPSA, Antibody; Rabbit anti-RPSA Antibody, Affinity Purified; SA; LAMR1; LBP/p40; LRP; LRP/LR; multidrug resistance-associated protein MGr1-Ag; laminin-binding protein precursor p40; p40; lamR; small ribosomal subunit protein uS2; NEM/1CHD4; 37LRP; 37 kDa laminin receptor; LBP; 37/67 kDa laminin receptor; laminin receptor 1 (67kD, ribosomal protein SA); 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; colon carcinoma laminin-binding protein; ICAS; LAMBR; Laminin receptor 1; 37 kDa laminin receptor precursor; ribosomal protein SA; anti-RPSA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPSA antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Zebrafish, Chicken
Immunogen
Between 245 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RPSA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPSA antibody AAA213202 (lot AAA213202-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse RPSA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPSA antibody AAA213202 (lot AAA213202-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPSA antibody AAA213202 (lot AAA213202-1) used for IP at 6 ug per reaction. RPSA was also immunoprecipitated by rabbit anti-RPSA antibody For blotting immunoprecipitated RPSA, AAA213202 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPSA antibody AAA213202 (lot AAA213202-1) used for IP at 6 ug per reaction. RPSA was also immunoprecipitated by rabbit anti-RPSA antibody For blotting immunoprecipitated RPSA, AAA213202 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-RPSA antibody
RPSA is required for the assembly and/or stability of the 40S ribosomal subunit. RPSA is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. RPSA also functions as a cell surface receptor for laminin and plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. RPSA may play a role in cell fate determination and tissue morphogenesis. It acts as a PPP1R16B-dependent substrate of PPP1CA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08865].
Product Categories/Family for anti-RPSA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,854 Da
NCBI Official Full Name
Homo sapiens ribosomal protein SA (RPSA), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ribosomal protein SA
NCBI Official Symbol
RPSA
NCBI Official Synonym Symbols
SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
NCBI Protein Information
40S ribosomal protein SA; 37 kDa laminin receptor; 37/67 kDa laminin receptor; colon carcinoma laminin-binding protein; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein precursor p40; multidrug resistance-associated protein MGr1-Ag
UniProt Protein Name
40S ribosomal protein SA
UniProt Gene Name
RPSA
UniProt Entry Name
RSSA_HUMAN
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Product Notes
The RPSA rpsa (Catalog #AAA213202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPSA Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPSA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RPSA rpsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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