<
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit LC3B Monoclonal Antibody | anti-LC3B antibody
[KO Validated] LC3B Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
LC3B; Monoclonal Antibody; [KO Validated] LC3B Rabbit mAb; MAP1LC3B; ATG8F; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; anti-LC3B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Applicable Applications for anti-LC3B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Immunogen
A synthetic peptide of human LC3B.
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Positive Samples
293T, C6, NIH/3T3
Cellular Location
Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37 degree C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37 degree C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.)
Related Product Information for anti-LC3B antibody
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Calculated MW 14kDa, 16kDa; Observed MW 14kDa, 16kDa
Similar Products
Product Notes
The LC3B (Catalog #AAA28305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] LC3B Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LC3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200. Researchers should empirically determine the suitability of the LC3B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LC3B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
