Application Data
(SDS page analysis of BBX-1H1 The antibody was purified by protein G affinity chromatography from cell culture supernatants Fig.2: SDS-PAGE analysis of purified BBX-1H1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BBX-1H1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Rat anti-Human LGR4 Monoclonal Antibody | anti-LGR4 antibody
Anti-LGR4 (GPR48) monoclonal antibody
Gene Names
LGR4; GPR48; BNMD17
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
LGR4, Antibody; Anti-LGR4 (GPR48) monoclonal antibody; GPR48; anti-LGR4 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
BBX-1H1
Specificity
anti-human LGR4
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
951
Applicable Applications for anti-LGR4 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human LGR4
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(SDS page analysis of BBX-1H1 The antibody was purified by protein G affinity chromatography from cell culture supernatants Fig.2: SDS-PAGE analysis of purified BBX-1H1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BBX-1H1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS page analysis of BBX-1H1 The antibody was purified by protein G affinity chromatography from cell culture supernatants Fig.2: SDS-PAGE analysis of purified BBX-1H1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BBX-1H1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using BBX-1H1 Cat.# GM- 0606. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR4-hum transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using BBX-1H1 Cat.# GM- 0606. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR4-hum transfected cells.)
Related Product Information for anti-LGR4 antibody
Background: LGR4 (Leucine-rich repeat- containing G protein-coupled receptor 4) belongs to the superfamily of G protein-coupled receptors (GPCRs). It is a 951 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are important for interaction with the glycoprotein ligands, and 7 transmembrane domains (1,3). LGR4 is highly expressed in the adult human pancreas but also with moderate levels of expression in placenta, kidney, brain and heart (2). LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. It is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression.
References
Hsu SY, Liang SG, Hsueh AJ (1998). Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G proteincoupled, seven-transmembrane region. Mol Endocrinol. 12(12):1830-45
Loh ED, Broussard SR, Liu Q, Copeland NG, Gilbert DJ, Jenkins NA, Kolakowski, LF Jr.(2000). Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet Cell Genet. 89(1-2):2-5
Loh ED, Broussard SR, Kolakowski LF (2001). Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem Biophys Res Commun. 282(3):757-64
Loh ED, Broussard SR, Liu Q, Copeland NG, Gilbert DJ, Jenkins NA, Kolakowski, LF Jr.(2000). Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet Cell Genet. 89(1-2):2-5
Loh ED, Broussard SR, Kolakowski LF (2001). Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem Biophys Res Commun. 282(3):757-64
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,667 Da
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 4
NCBI Official Synonym Full Names
leucine-rich repeat containing G protein-coupled receptor 4
NCBI Official Symbol
LGR4
NCBI Official Synonym Symbols
GPR48; BNMD17
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 4; G protein-coupled receptor 48
UniProt Gene Name
LGR4
UniProt Synonym Gene Names
GPR48
UniProt Entry Name
LGR4_HUMAN
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Product Notes
The LGR4 lgr4 (Catalog #AAA71462) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-LGR4 (GPR48) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LGR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the LGR4 lgr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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