FCM/FACS (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with (red line) at 1?50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
LGR5 recombinant antibody
LGR5 Antibody
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, Rat
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
LGR5, Recombinant Antibody; LGR5 Antibody; Leucine-rich repeat-containing G-protein coupled receptor 5 (G-protein coupled receptor 49) (G-protein coupled receptor 67) (G-protein coupled receptor HG38); LGR5; GPR49 GPR67; LGR5 recombinant antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
4H8
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for LGR5 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human LGR5/GPR49
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with (red line) at 1?50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with (red line) at 1?50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissueAll lanes: LGR5 antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 100, 98, 93 kDaObserved band size: 100 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissueAll lanes: LGR5 antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 100, 98, 93 kDaObserved band size: 100 kDa)
Related Product Information for LGR5 recombinant antibody
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Product Categories/Family for LGR5 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99998 MW
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
NCBI Official Synonym Full Names
leucine rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
LGR5
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Gene Name
LGR5
UniProt Synonym Gene Names
GPR49; GPR67
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Product Notes
The LGR5 lgr5 (Catalog #AAA243879) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGR5 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LGR5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LGR5 lgr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR5, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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