WB (Western Blot)
(Western blot analysis of LGR5 expression in K562 cell line lysates; the lane is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse LGR5 Polyclonal Antibody | anti-LGR5 antibody
LGR5 Antibody
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LGR5, Antibody; LGR5 Antibody; FEX; G protein coupled receptor 49; G protein coupled receptor 67; g protein-coupled receptor fex; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; GRP 49; GRP49; HG 38; HG38; Leucine rich repeat containing G protein coupled receptor 5; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; LGR5; LGR5_HUMAN; MGC117008; Orphan G protein coupled receptor HG38; anti-LGR5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
LGR5 antibody detects endogenous levels of total LGR5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
883
Applicable Applications for anti-LGR5 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human LGR5, corresponding to a region within the internal amino acids.
WB (Western Blot)
(Western blot analysis of LGR5 expression in K562 cell line lysates; the lane is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of LGR5 expression in K562 cell line lysates; the lane is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry)
(AAA323611 at 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA323611 at 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323611 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323611 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-LGR5 antibody
Description: Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100 kDa
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
NCBI Official Synonym Full Names
leucine rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
LGR5
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Gene Name
LGR5
UniProt Synonym Gene Names
GPR49; GPR67
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Product Notes
The LGR5 lgr5 (Catalog #AAA323611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGR5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGR5 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the LGR5 lgr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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