WB (Western Blot)
(Western blot analysis of LYVE1 on di ferent lysates with Rabbit anti-LYVE1 antibody at 1/2,000 dilution.Lane 1: MCF-7 ce l lysate.Lane 2: SW480 ce l lysate.Lane 3: A431 ce l lysate.Lane 4: HUVEC ce l lysate. Lysates/proteins at 10 ug/Lane.Predicted band size: 35 kDa. Observed band size: 35 kDa.Exposure time: 2 minutes; 10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
Rabbit anti-Human LYVE1 Monoclonal Antibody | anti-LYVE1 antibody
LYVE1
Gene Names
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
Reactivity
Human
Applications
Western Blot
Purity
Protein A a finity purified.
Synonyms
LYVE1, Antibody; LYVE1; Lymphatic vessel endothelial hyaluronic acid receptor 1; Cell surface retention sequence-binding protein 1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor; anti-LYVE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
JF0979
Purity/Purification
Protein A a finity purified.
Form/Format
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
322
Applicable Applications for anti-LYVE1 antibody
WB (Western Blot)
Immunogen
Synthetic peptide within Human LYVE1 aa 36-72 / 322.
Positive control
MCF-7 ce l lysate, SW480 ce l lysate, A431 ce l lysate, HUVEC ce l lysate, A431, HUVEC,SW480.
Subcellular location
Membrane.
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles
WB (Western Blot)
(Western blot analysis of LYVE1 on di ferent lysates with Rabbit anti-LYVE1 antibody at 1/2,000 dilution.Lane 1: MCF-7 ce l lysate.Lane 2: SW480 ce l lysate.Lane 3: A431 ce l lysate.Lane 4: HUVEC ce l lysate. Lysates/proteins at 10 ug/Lane.Predicted band size: 35 kDa. Observed band size: 35 kDa.Exposure time: 2 minutes; 10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
WB (Western Blot)
(Western blot analysis of LYVE1 on di ferent lysates with Rabbit anti-LYVE1 antibody at 1/2,000 dilution.Lane 1: MCF-7 ce l lysate.Lane 2: SW480 ce l lysate.Lane 3: A431 ce l lysate.Lane 4: HUVEC ce l lysate. Lysates/proteins at 10 ug/Lane.Predicted band size: 35 kDa. Observed band size: 35 kDa.Exposure time: 2 minutes; 10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
Related Product Information for anti-LYVE1 antibody
Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) is expressed on the cell surface as a protein that is reduced by glycosidase treatment. LYVE-1 is abundant in spleen,lymph node, heart, lung and fetal liver, and is less abundant in appendix, bone marrow,placenta, muscle and adult liver. Expression of LYVE-1 is largely restricted to endothelial cells lining lymphatic vessels and splenic sinusoidal endothelial ce ls. LYVE-1 binds to both soluble and immobilized hyaluronan (HA) with greater specificity than HCAM. Like HCAM,the LYVE-1 molecule binds both soluble and immobilized HA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted band size: 35 kDa
NCBI Official Full Name
lymphatic vessel endothelial hyaluronic acid receptor 1
NCBI Official Synonym Full Names
lymphatic vessel endothelial hyaluronan receptor 1
NCBI Official Symbol
LYVE1
NCBI Official Synonym Symbols
HAR; XLKD1; LYVE-1; CRSBP-1
NCBI Protein Information
lymphatic vessel endothelial hyaluronic acid receptor 1
UniProt Protein Name
Lymphatic vessel endothelial hyaluronic acid receptor 1
UniProt Gene Name
LYVE1
UniProt Synonym Gene Names
CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
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Product Notes
The LYVE1 lyve1 (Catalog #AAA268409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LYVE1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LYVE1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the LYVE1 lyve1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYVE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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