FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit MARK2 Monoclonal Antibody | anti-MARK2 antibody
MARK2 Antibody
Gene Names
MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
MARK2, Antibody; MARK2 Antibody; ELKL motif kinase 1 antibody; ELKL motif kinase antibody; EMK-1 antibody; EMK1 antibody; MAP/microtubule affinity regulating kinase 2 antibody; MAP/microtubule affinity-regulating kinase 2 antibody; Mark2 antibody; MARK2_HUMAN antibody; MGC99619 antibody; PAR 1 antibody; Par 1b antibody; PAR1 homolog antibody; Par1b antibody; Ser/Thr protein kinase PAR 1B antibody; Serine/threonine protein kinase EMK antibody; Serine/threonine protein kinase MARK2 antibody; Serine/threonine-protein kinase MARK2 antibody; anti-MARK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JE40-97
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MARK2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein near the C-terminus of human MARK2.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MARK2 on MCF-7 (1) and SK-Br-3 (2) cell lysate using anti-MARK2 antibody at 1/2, 000 dilution.)
WB (Western Blot)
(Western blot analysis of MARK2 on MCF-7 (1) and SK-Br-3 (2) cell lysate using anti-MARK2 antibody at 1/2, 000 dilution.)
Related Product Information for anti-MARK2 antibody
Microtubule affinity-regulating kinase 2 (MARK2), also known as EMK1 (ELKL motif kinase 1) or Par1b, is a 788 amino acid protein that is a member of the protein kinase superfamily, MARK subfamily. Highly expressed in heart, brain, skeletal muscle and pancreas, MARK2 is essential for the asymmetric development of membrane domains around polarized epithelial cells. Activation of MARK2 by phosphorylation on Thr 208 allows the protein to modulate the building of a columnar versus a hepatic epithelial cell. MARK2 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. MARK2 is expressed as 14 isoforms produced by alternative splicing events. Some of these isoforms may function in graft rejection.
Product Categories/Family for anti-MARK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,911 Da
NCBI Official Full Name
serine/threonine-protein kinase MARK2 isoform d
NCBI Official Synonym Full Names
MAP/microtubule affinity-regulating kinase 2
NCBI Official Symbol
MARK2
NCBI Official Synonym Symbols
EMK1; EMK-1; PAR-1; Par1b; Par-1b
NCBI Protein Information
serine/threonine-protein kinase MARK2; PAR1 homolog b; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK
UniProt Protein Name
Serine/threonine-protein kinase MARK2
UniProt Gene Name
MARK2
UniProt Synonym Gene Names
EMK1; EMK-1; Par-1b; Par1b
UniProt Entry Name
MARK2_HUMAN
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Product Notes
The MARK2 mark2 (Catalog #AAA312696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARK2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MARK2 mark2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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