Rabbit anti-Human MAX Monoclonal Antibody | anti-MAX antibody

Recombinant Anti-MAX Antibody, Rabbit Monoclonal

Cat. Num. AAA255613

• Gene Names: MAX; bHLHd4
• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Protein A
• Synonyms: MAX, Antibody; Recombinant Anti-MAX Antibody, Rabbit Monoclonal; MAX/MYC associated factor X Antibody, Rabbit MAb; MYC associated factor X; Anti-bHLHd4 Antibody; anti-MAX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 103
• Specificity: Human MAX/MYC associated factor X
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAX antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human MAX/MYC associated factor X protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human MAX/MYC associated factor X (rh MAX/MYC associated factor X).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-MAX rabbit monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(MAX was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human MAX expression on Raji cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.)

Related Product Information for anti-MAX antibody

MYC associated factor X contains 1 basic helix-loop-helix (bHLH) domain and belongs to the MAX family. It is highly expressed in the brain, heart, and lung while lower levels are seen in the liver, kidney, and skeletal muscle. MYC associated factor X can form homodimers and heterodimers with other family members, which include Mad, Mxi1, and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation, and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. MYC associated factor X may also repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Multiple alternatively spliced transcript variants have been described for MYC associated factor X gene but the full-length nature for some of them is unknown.

Product Categories/Family for anti-MAX antibody

Apoptosis Transcription Factors and Regulators; P38 MAPK Signaling Pathway

References

Mac Partlin M, et al. (2003) Interactions of the DNA mismatch repair proteins MLH1 and MSH2 with c-MYC and MAX. Oncogene. 22(6):819-25.Cheng SW, et al. (1999) c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function. Nat enet. 22(1):102-5.McMahon SB, et al. (1998) The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins. Cell. 94(3):363-74.

NCBI and Uniprot Product Information

• NCBI GI #: 21704261
• NCBI GeneID: 4149
• NCBI Accession #: NP_002373
• NCBI GenBank Nucleotide #: NM_002382.4
• UniProt Accession #: P61244; P25912; P52163; Q14803; Q96CY8; A6NH73; A8K265; A8K4G4; A8K824
• Molecular Weight: 19.3 kDa (168aa), confirmed by MALDI-TOF
• NCBI Official Full Name: protein max isoform a
• NCBI Official Synonym Full Names: MYC associated factor X
• NCBI Official Symbol: MAX
• NCBI Official Synonym Symbols: bHLHd4
• NCBI Protein Information: protein max
• UniProt Protein Name: Protein max
• UniProt Gene Name: MAX
• UniProt Synonym Gene Names: BHLHD4; bHLHd4
• UniProt Entry Name: MAX_HUMAN