WB (Western Blot)
(Western Blotusinganti-MHCIantibodyR1-21.2(AAA72498) Mousethymustissuelysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofR1-21.2(AAA72498)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
MHC I Recombinant Antibody | MHCI recombinant antibody
Anti-MHC I [R1-21.2], Goat IgG, kappa
Reactivity
Mouse
Applications
immunoassay, Radioimmunoassay, Immunoprecipitation, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
MHC I, Recombinant Antibody; Anti-MHC I [R1-21.2], Goat IgG, kappa; MHC I; Anti-MHC I [R1-21.2]; R1.21.2; H-2; MHC-I; MHC Class I; MHCI recombinant antibody
Host
Goat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Goat IgG kappa
Clone Number
R1-21.2
Specificity
This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for MHCI recombinant antibody
RIA (Radioimmunoassay), IP (Immunoprecipitation), IHC (Immunohistochemistry)
Chimeric Use Statement
This chimeric goat antibody was made using the variable domain sequences of the original rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blotusinganti-MHCIantibodyR1-21.2(AAA72498) Mousethymustissuelysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofR1-21.2(AAA72498)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-MHCIantibodyR1-21.2(AAA72498) Mousethymustissuelysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofR1-21.2(AAA72498)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescencestainingofmousesplenocytesusinganti-MHCIantibody(AAA72498)R1-21.2. ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytesimmobilizedonShi-fixcover-slipsandstainedwiththechimericrabbitIgGversionofR1-21.2(AAA72498)at10ug/mlfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaininginasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72498,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithanti-FluoresceinantibodyfollowedbyAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingofmousesplenocytesusinganti-MHCIantibody(AAA72498)R1-21.2. ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytesimmobilizedonShi-fixcover-slipsandstainedwiththechimericrabbitIgGversionofR1-21.2(AAA72498)at10ug/mlfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaininginasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72498,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithanti-FluoresceinantibodyfollowedbyAlexaFluor488secondaryantibody.)
Related Product Information for MHCI recombinant antibody
This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
References
Koch et al Comparison of allogeneic and xenogeneic determinants on the H-2Kk molecule Transplantation, August 1983, Vol.36(2), pp.177-80 [Peer Reviewed Journal] N/A6192569
Note on publication:
Describes the original generation and characterisation of this antibody.
Note on publication:
Describes the original generation and characterisation of this antibody.
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Product Notes
The MHCI (Catalog #AAA72498) is a Recombinant Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-MHC I [R1-21.2], Goat IgG, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC I can be used in a range of immunoassay formats including, but not limited to, RIA (Radioimmunoassay), IP (Immunoprecipitation), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MHCI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC I, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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