IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-MHCI(RT1A)antibodyOX-18(AAA72457). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-18(AAA72457)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72457,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
MHC I (RT1A) Recombinant Antibody | MHCI recombinant antibody
Anti-MHC I (RT1A) [OX-18], Rabbit IgG, kappa
Gene Names
RT1-EC2; RTS; RT1-A; MHCRTA; RT1.Al; RT1Aw2; RT1-Aw2; RT1.EC2; RATMHCRTA
Reactivity
Rat
Applications
Immunohistochemistry, ELISA, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
MHC I (RT1A), Recombinant Antibody; Anti-MHC I (RT1A) [OX-18], Rabbit IgG, kappa; MHC I (RT1A); Anti-MHC I (RT1A) [OX-18]; RT-1A; RT-1A histocompatibility antigen; non-polymorphic determinant of rat MHC class I; MHC class I; OX18; MRC OX18; MRC OX-18; MHCI recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-18
Specificity
OX18 binds to a non-polymorphic/monomorphic determinant of rat MHC class I protein.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for MHCI recombinant antibody
IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-MHCI(RT1A)antibodyOX-18(AAA72457). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-18(AAA72457)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72457,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-MHCI(RT1A)antibodyOX-18(AAA72457). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-18(AAA72457)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72457,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-MHCI(RT1A)antibodyOX-18(AAA72457). Ratlymphnodetissuelysates(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofOX-18(AAA72457)at0.01ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-MHCI(RT1A)antibodyOX-18(AAA72457). Ratlymphnodetissuelysates(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofOX-18(AAA72457)at0.01ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-MHCI(RT1A)antibodyOX-18(AAA72457). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-18(AAA72457,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-MHCI(RT1A)antibodyOX-18(AAA72457). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-18(AAA72457,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for MHCI recombinant antibody
OX-18 was prepared by immunizing BALB/c mice with rat spleen glycoproteins of the size of Ia antigens which has been depleted of Ia antigens using an OX-6 affinity column.
References
Fukumoto T, McMaster WR, Williams AF Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982 Mar;12(3):237-43 N/A6178598
Note on publication:
Describes the generation of the original hybridoma and the use of OX18 in analyzing RT-1A antigens on rat thymocytes
Note on publication:
Describes the generation of the original hybridoma and the use of OX18 in analyzing RT-1A antigens on rat thymocytes
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,830 Da
NCBI Official Full Name
RT1 class I histocompatibility antigen, AA alpha chain
NCBI Official Synonym Full Names
RT1 class Ib, locus EC2
NCBI Official Symbol
RT1-EC2
NCBI Official Synonym Symbols
RTS; RT1-A; MHCRTA; RT1.Al; RT1Aw2; RT1-Aw2; RT1.EC2; RATMHCRTA
NCBI Protein Information
class I histocompatibility antigen, Non-RT1.A alpha-1 chain; MHC class I antigen; MHC class Ia protein; RT1 class Ib gene RT1Aw2; RT1 class Ib gene(Aw2); RT1 class Ib, locus Aw2
UniProt Protein Name
RT1 class I histocompatibility antigen, AA alpha chain
UniProt Entry Name
HA12_RAT
Similar Products
Product Notes
The MHCI (Catalog #AAA72457) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MHC I (RT1A) [OX-18], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MHC I (RT1A) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the MHCI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC I (RT1A), Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.