WB (Western Blot)
(Western Blotusinganti-MHCclassII(CD74)antibodyOX-6(AAA72438) Ratspleen(A),ratthymus(B)andratlymphnode(C)lysatesamples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-6(AAA72438)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD74is~24.5-31.6kDa,dependingontheisoformandpost-translationalmodification.AAA72438successfullydetectedCD74inratspleen,thymusandlymphnodelysates.)
MHC II Recombinant Antibody | MHCII recombinant antibody
Anti-MHC II [OX-6], Mouse IgG1, kappa
Reactivity
Rat, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunoprecipitation, immunoassay, Radioimmunoassay
Purity
Protein A affinity purified
Synonyms
MHC II, Recombinant Antibody; Anti-MHC II [OX-6], Mouse IgG1, kappa; MHC II; Anti-MHC II [OX-6]; MHC Class II; MRC OX-6; CD74; MRC OX 6; Major Histocompatibility complex II; MHCII recombinant antibody
Host
Mouse
Reactivity
Rat, Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
OX-6
Specificity
This antibody recognises a monomorphic determinant of the rat MHC II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s]
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for MHCII recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IP (Immunoprecipitation), RIA (Radioimmunoassay)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blotusinganti-MHCclassII(CD74)antibodyOX-6(AAA72438) Ratspleen(A),ratthymus(B)andratlymphnode(C)lysatesamples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-6(AAA72438)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD74is~24.5-31.6kDa,dependingontheisoformandpost-translationalmodification.AAA72438successfullydetectedCD74inratspleen,thymusandlymphnodelysates.)
WB (Western Blot)
(Western Blotusinganti-MHCclassII(CD74)antibodyOX-6(AAA72438) Ratspleen(A),ratthymus(B)andratlymphnode(C)lysatesamples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-6(AAA72438)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD74is~24.5-31.6kDa,dependingontheisoformandpost-translationalmodification.AAA72438successfullydetectedCD74inratspleen,thymusandlymphnodelysates.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocytescellswithanti-MHCclassII(CD74)antibodyOX-6(AAA72438) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-6(AAA72438)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstainingofasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72438,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocytescellswithanti-MHCclassII(CD74)antibodyOX-6(AAA72438) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-6(AAA72438)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandsomecytoplasmicstainingofasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72438,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-MHCclassIIantibodyOX-6(AAA72438) Stainingofrat(Rattusnorvegicus)leukocyteswitheitheranisotypecontrol(A)ortherabbit-chimericversionofOX-6(B,AAA72438)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusinganAlexaFluor488conjugateddonkeyanti-rabbitantibody(ab150073)andcellsanalysedonaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-MHCclassIIantibodyOX-6(AAA72438) Stainingofrat(Rattusnorvegicus)leukocyteswitheitheranisotypecontrol(A)ortherabbit-chimericversionofOX-6(B,AAA72438)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusinganAlexaFluor488conjugateddonkeyanti-rabbitantibody(ab150073)andcellsanalysedonaFACSCantoflow-cytometer.)
Related Product Information for MHCII recombinant antibody
This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice.
References
McMaster et al. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. European Journal of Immunology. 1979, 9: 426-433 N/A315315
Note on publication:
Describes the original use of this antibody to identify and purify rat Ia glycoproteins, using indirect binding assays, FC and IP.
Note on publication:
Describes the original use of this antibody to identify and purify rat Ia glycoproteins, using indirect binding assays, FC and IP.
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Product Notes
The MHCII (Catalog #AAA72438) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MHC II [OX-6], Mouse IgG1, kappa reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC II can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IP (Immunoprecipitation), RIA (Radioimmunoassay). Researchers should empirically determine the suitability of the MHCII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC II, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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