FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MIMIT with M25103 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human MIMIT Monoclonal Antibody | anti-MIMIT antibody
MIMIT Mouse mAb
Gene Names
NDUFAF2; MMTN; B17.2L; mimitin; NDUFA12L
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
MIMIT, Antibody; MIMIT Mouse mAb; Mimitin /NDUFAF2 Mouse mAb; B17.2-like; B17.2L; B172-like; B172L; Mimitin; Mimitin, mitochondrial; mitochondrial; MMTN; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase (ubiquinone) complex I, assembly factor 2; NADH dehydrogenase 1 alpha subcomplex assembly factor 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein; NDUFA12L; NDUFAF2; mimitin; anti-MIMIT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
169
Applicable Applications for anti-MIMIT antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human MIMIT
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MIMIT with M25103 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MIMIT with M25103 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation)
(MIMIT was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25103 of 10 ug. Western blot was performed from the immunopre-cipitate using M25103 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 20 KDaObserved band size : 20 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
IP (Immunoprecipitation)
(MIMIT was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25103 of 10 ug. Western blot was performed from the immunopre-cipitate using M25103 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 20 KDaObserved band size : 20 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-MIMIT antibody
NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency.
Product Categories/Family for anti-MIMIT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 20kDa
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase complex assembly factor 2
NCBI Official Symbol
NDUFAF2
NCBI Official Synonym Symbols
MMTN; B17.2L; mimitin; NDUFA12L
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
UniProt Gene Name
NDUFAF2
UniProt Synonym Gene Names
NDUFA12L; MMTN1 PublicationManual assertion based on opinion iniRef.1
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Product Notes
The MIMIT ndufaf2 (Catalog #AAA62378) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MIMIT Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIMIT can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MIMIT ndufaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIMIT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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