IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 antibody.)
Mouse MLH1 Monoclonal Antibody | anti-MLH1 antibody
MLH1 antibody
Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
MLH1, Antibody; MLH1 antibody; Mouse monoclonal MLH1 antibody; Monoclonal MLH1 antibody; Anti-MLH1 antibody; Mutl Homolog 1 Colon Cancer Nonpolyposis Type 2 antibody; COCA2 antibody; FCC2 antibody; HNPCC antibody; HNPCC2 antibody; MGC5172 antibody; hMLH1 antibody antibody; MLH1; MLH 1; MLH-1; MLH 1 antibody; MLH-1 antibody; anti-MLH1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C9C7
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
34
Applicable Applications for anti-MLH1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Immunogen
MLH1 antibody was raised in Mouse using a purified recombinant fragment of MLH1 (aa381-483) expressed in E Coli as the immunogen.
Cross-Reactivity
Human, Monkey
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 antibody.)
WB (Western Blot)
(Western Blot showing MLH1 antibody used against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
WB (Western Blot)
(Western Blot showing MLH1 antibody used against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of Hela cells using MLH1 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of Hela cells using MLH1 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Related Product Information for anti-MLH1 antibody
MLH1 is a human homolog of the E Coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed inlymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid andgall bladder, and is methylated in several ovarian tumors.
Product Categories/Family for anti-MLH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
73,837 Da
NCBI Official Full Name
MLH1, partial
NCBI Official Synonym Full Names
mutL homolog 1
NCBI Official Symbol
MLH1
NCBI Official Synonym Symbols
FCC2; COCA2; HNPCC; hMLH1; HNPCC2
NCBI Protein Information
DNA mismatch repair protein Mlh1
UniProt Protein Name
DNA mismatch repair protein Mlh1
UniProt Gene Name
MLH1
UniProt Synonym Gene Names
COCA2
Similar Products
Product Notes
The MLH1 mlh1 (Catalog #AAA225001) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the MLH1 mlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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