Mouse MLH1 Monoclonal Antibody | anti-MLH1 antibody

MLH1

Cat. Num. AAA59227

• Gene Names: Mlh1; CG11482; DmelCG11482; dmlh-1; dmlh1
• Reactivity: Human, Rat, Mouse
• Synonyms: MLH1, Antibody; MLH1; anti-MLH1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: G168-728

• Immunogen: Full length recombinant MLH1 protein.
• Control: Testis, Tonsil, Colon, Kidney, Colon Carcinoma
• Localization: Nuclear
• Intended Uses: This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations.
• Presentation: Anti-MLH1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.; 3. Dispose of unused solution with copious amounts of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information refer to Safety Data Sheet for this product.; 8. For complete recommendations for handling biological specimens, please refer to the CDC document, "Guidelines for Safe Work Practices in Human and AMDL (see References in this document).
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter (please inquire) or organic solvent based resin such as PermaMounter (please inquire).
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
• Preparation and Storage: Store at 2 to 8 degree C.

IHC (Immunohistochemistry)

IHC (Immunohiostchemistry)

(MLH1 Antibody Immunohistochemistry on a FFPE Colon Carcinoma Tissue)

Dilution Info

Related Product Information for anti-MLH1 antibody

MLH1 is a mismatch repair gene of around 87 kDa, commonly associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC). This gene was identified as a locus frequently mutated in HNPCC. It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. In a high proportion of patients with microsatellite instability (MSI-H), the MLH1 protein is typically deficient. This protein deficiency is linked to the autosomal dominant condition of Hereditary Non-Polyposis Colon Cancer. The anti-MLH1antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite-unstable have a better prognosis than their microsatellite stable counterparts.

NCBI and Uniprot Product Information

• NCBI GI #: 17136968
• NCBI GeneID: 35796
• NCBI Accession #: NP_477022.1
• NCBI GenBank Nucleotide #: NM_057674.2
• NCBI Official Full Name: Mlh1
• NCBI Official Symbol: Mlh1
• NCBI Official Synonym Symbols: CG11482; DmelCG11482; dmlh-1; dmlh1
• NCBI Protein Information: CG11482 gene product from transcript CG11482-RA; Mlh1; CG11482-PA; Mlh1-PA