FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human MMP2 expression on MG63 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human MMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human MMP-2 Monoclonal Antibody | anti-MMP2 antibody
Anti-MMP-2 Antibody, Mouse Monoclonal
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Protein A
Synonyms
MMP-2, Antibody; Anti-MMP-2 Antibody, Mouse Monoclonal; MMP-2 Antibody, Mouse MAb; matrix metallopeptidase 2; Anti-CLG4 Antibody; Anti-CLG4A Antibody; Anti-MMP-2 Antibody; Anti-MMP-II Antibody; Anti-MONA Antibody; Anti-TBE-1 Antibody; anti-MMP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
05
Specificity
Human MMP-2.
No cross-reactivity in ELISA with Human MMP1, Human MMP7, Human MMP9, Human cell lysate (293 cell line)
No cross-reactivity in ELISA with Human MMP1, Human MMP7, Human MMP9, Human cell lysate (293 cell line)
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MMP2 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human MMP-2 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MMP-2 (rh MMP-2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human MMP2 expression on MG63 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human MMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human MMP2 expression on MG63 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human MMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
WB (Western Blot)
(Anti-MMP2 mouse monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:74 kDaObserved band size:55 kDa)
WB (Western Blot)
(Anti-MMP2 mouse monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:74 kDaObserved band size:55 kDa)
Related Product Information for anti-MMP2 antibody
Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
Product Categories/Family for anti-MMP2 antibody
References
Thompson EW, et al. (1994) Collagen induced MMP-2 activation in human breast cancer. Breast Cancer Res Treat. 31(2-3): 357-70.Jezierska A, et al. (2009) Matrix metalloproteinase-2 involvement in breast cancer progression: a mini-review. Med Sci Monit. 15(2): RA32-40.Fredrich M, et al. (2010) MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport. 21(5): 324-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,831 Da
NCBI Official Full Name
72 kDa type IV collagenase isoform 1 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
NCBI Official Symbol
MMP2
NCBI Official Synonym Symbols
CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
NCBI Protein Information
72 kDa type IV collagenase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II
UniProt Protein Name
72 kDa type IV collagenase
UniProt Gene Name
MMP2
UniProt Synonym Gene Names
CLG4A; MMP-2
UniProt Entry Name
MMP2_HUMAN
Similar Products
Product Notes
The MMP2 mmp2 (Catalog #AAA254359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MMP-2 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MMP-2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MMP2 mmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.