FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit MMP9 Monoclonal Antibody | anti-MMP9 antibody
MMP9 Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining MMP9 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MMP9 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1, 000 dilution.)
NCBI and Uniprot Product Information
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Product Notes
The MMP9 mmp9 (Catalog #AAA30359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP9 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the MMP9 mmp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What does a rabbit monoclonal antibody mean in research applications?
A rabbit monoclonal antibody is produced by fusing B-cells from an immunized rabbit with a specialized myeloma cell line. In research, these antibodies are prized for their high affinity and exceptional specificity. Rabbits possess a unique immune system that generates a more diverse range of antibodies than mice, often resulting in better recognition of small epitopes or subtle post-translational modifications, making them ideal for sensitive assays.
What biological processes is MMP-9 commonly used as a marker for?
MMP-9 (Matrix Metalloproteinase-9) serves as a key marker for extracellular matrix (ECM) remodeling, tissue repair, and inflammation. It is an enzyme responsible for degrading components like gelatin and collagen. Elevated MMP-9 levels are frequently associated with physiological processes such as wound healing and bone resorption, as well as pathological states including tumor invasion, angiogenesis, and various cardiovascular diseases, where the structural integrity of tissues is being altered.
How is a rabbit monoclonal antibody different from a mouse monoclonal antibody?
The primary difference lies in the host species and the resulting antibody characteristics. Rabbit antibodies generally offer higher affinity and recognize a wider variety of epitopes compared to mouse monoclonals. Additionally, because rabbits are evolutionarily distant from humans and mice, they can produce antibodies against antigens that might be poorly immunogenic in rodents. AAA Biotech offers rabbit monoclonals to provide researchers with higher sensitivity and lower background noise in complex assays.
What are the main types of monoclonal antibodies used in laboratories?
Laboratories primarily use mouse and rabbit monoclonal antibodies, though rat and hamster versions are also available. These are categorized by their isotype, with IgG being the most common for research applications like Western blotting and IHC. Modern labs also utilize recombinant monoclonal antibodies, which are produced in vitro using defined genetic sequences. This ensures high batch-to-batch consistency and eliminates the variability often associated with traditional hybridoma-based production methods.
Why is MMP-9 studied in cancer, inflammation, or tissue remodeling research?
MMP-9 is studied in these fields because of its ability to break down the basement membrane and extracellular matrix. In cancer, this degradation facilitates tumor cell migration and metastasis. In inflammatory diseases, MMP-9 aids the movement of white blood cells to injury sites. Understanding MMP-9 activity helps researchers at AAA Biotech and global institutions develop strategies to control excessive tissue breakdown, which is a hallmark of chronic inflammation and invasive cancers.
Can a rabbit MMP9 monoclonal antibody be used for Western blot or IHC?
Yes, this rabbit monoclonal antibody is highly versatile and validated for both Western blot (WB) and immunohistochemistry (IHC). For WB, it provides a sensitive method to quantify MMP-9 protein levels in secreted fluids or cell lysates. In IHC, it enables the precise localization of the enzyme within tissue sections, helping researchers identify which specific cells are producing MMP-9 during inflammation or tumor growth, ensuring reliable data across different experimental platforms.
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