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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using MTAP Mouse Monoclonal Antibody (MTAP/1813).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse MTAP Monoclonal Antibody | anti-MTAP antibody
MTAP (Tumor Suppressor Marker)
Gene Names
MTAP; BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Western Blot, ELISA
Synonyms
MTAP, Antibody; MTAP (Tumor Suppressor Marker); BDMF; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAPase; S-methyl-5''-thioadenosine phosphorylase; anti-MTAP antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MTAP/1813
Specificity
Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
283
Applicable Applications for anti-MTAP antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human MTAP protein fragment (aa97-196)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
9p21.3
Positive Control
HepG2, A431, HeLa or MCF-7 cells. Kidney
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using MTAP Mouse Monoclonal Antibody (MTAP/1813).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using MTAP Mouse Monoclonal Antibody (MTAP/1813).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
WB (Western Blot)
(Western Blot Analysis of human A431 cell lysate using MTAP Mouse Monoclonal Antibody (MTAP/1813).)
WB (Western Blot)
(Western Blot Analysis of human A431 cell lysate using MTAP Mouse Monoclonal Antibody (MTAP/1813).)
SDS-PAGE
(SDS-PAGE Analysis Purified MTAP Mouse Monoclonal Antibody (MTAP/1813).Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified MTAP Mouse Monoclonal Antibody (MTAP/1813).Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Kidney stained with MTAP Mouse Monoclonal Antibody (MTAP/1813).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Kidney stained with MTAP Mouse Monoclonal Antibody (MTAP/1813).)
References
Nobori, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93: 6203-6208.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31kDa
NCBI Official Full Name
S-methyl-5'-thioadenosine phosphorylase
NCBI Official Synonym Full Names
methylthioadenosine phosphorylase
NCBI Official Symbol
MTAP
NCBI Official Synonym Symbols
BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
NCBI Protein Information
S-methyl-5'-thioadenosine phosphorylase
UniProt Protein Name
S-methyl-5'-thioadenosine phosphorylase
UniProt Gene Name
MTAP
UniProt Synonym Gene Names
MTA phosphorylase; MTAP; MTAPase
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Product Notes
The MTAP mtap (Catalog #AAA214669) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MTAP (Tumor Suppressor Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's MTAP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MTAP mtap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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