Mouse anti-Human MTDH Monoclonal Antibody | anti-MTDH antibody

MTDH Monoclonal Antibody

Cat. Num. AAA320166

• Gene Names: MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: MTDH, Antibody; MTDH Monoclonal Antibody; Protein LYRIC; MTDH; AEG1; LYRIC; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; anti-MTDH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: MTDH Monoclonal Antibody detects endogenous levels of MTDH protein.
• Purity/Purification: Affinity purification
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-MTDH antibody: ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Purified recombinant fragment of human MTDH expressed in E coli.
• Modification: Unmodified/Total
• Caution: Was originally (PubMed; 15093543) thought to be a type II membrane protein but this is inconsistent with the results of multiple phosphorylation studies because this topology would locate the phosphorylation sites in the lumen or extracellularly rather than in the cytoplasm.
• Function: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
• Induction: By TNF-alpha (at protein level). By HIV-1 infection of primary fetal astrocytes.
• Miscellaneous: Knockdown significantly reduces the adhesion of cancer cells to lung microvascular endothelial cells and the reciprocal effect is observed following overexpression.
• Subcellular Location: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
• Subunit: Interacts with BCIPP, CREBBP/CBP and RELA/p65.
• Tissue Specificity: Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
• Preparation and Storage: Store at -20 degree C/1 year

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Hela cells using MTDH Monoclonal Antibody (green) and negative control (purple).)

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis of paraffin-embedded human Liver tissues with AEC staining using MTDH Monoclonal Antibody.)

WB (Western Blot)

(Western Blot analysis using MTDH Monoclonal Antibody against K562 (1). SKBR-3 (2). T47D (3). HeLa (4) and MCF-7 (5) cell lysate.)

Product Categories/Family for anti-MTDH antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 223555917
• NCBI GeneID: 92140
• NCBI Accession #: NP_848927.2
• NCBI GenBank Nucleotide #: NM_178812.3
• UniProt Accession #: Q86UE4; Q05DH2; Q52QU9; Q6PK07; Q8TCX3
• Molecular Weight: 63,837 Da
• NCBI Official Full Name: protein LYRIC
• NCBI Official Synonym Full Names: metadherin
• NCBI Official Symbol: MTDH
• NCBI Official Synonym Symbols: 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• NCBI Protein Information: protein LYRIC
• UniProt Protein Name: Protein LYRIC
• UniProt Gene Name: MTDH
• UniProt Synonym Gene Names: AEG1; LYRIC; AEG-1
• UniProt Entry Name: LYRIC_HUMAN