anti-Human MUC1 (CD227) Monoclonal Antibody | anti-MUC1 antibody

Mouse anti MUC1 (CD227)

Cat. Num. AAA77502

• Gene Names: MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MCKD1; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunoblot, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Synonyms: MUC1 (CD227), Antibody; Mouse anti MUC1 (CD227); anti-MUC1 antibody

• Host: Mouse (Balb/c)
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 175C5
• Specificity: 175C5 shows a high preference for breast carcinomas relative to normal breast epithelium. The antibdy reacts with a carcinoma-associated antigen in both adenocarcinomas and squamous cell carcinomas of different origins.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-MUC1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), IB (Immunoblot), FCM/FACS (Flow Cytometry), ELISA, EM (Immunoelectron Microscopy)
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Related Product Information for anti-MUC1 antibody

Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.

Product Categories/Family for anti-MUC1 antibody

CD markers; Cell adhesion; Cancer; Cell adhesion

References

J. Hilkens, J. Wesseling, H. L. Vos, J. STorm, M. Boer, S. W. van der Valk and M. C. E. Maas, Episialin/CA15-3: its structure and involvement in breast cancer progression, Ned Tijdschr Klin Chem 1995; 20: 293-298

NCBI and Uniprot Product Information

• NCBI GI #: 67189069
• NCBI GeneID: 4582
• NCBI Accession #: NP_001018017.1
• NCBI GenBank Nucleotide #: NM_001018017.2
• UniProt Accession #: P15941; P13931; P15942; P17626; Q0VAP5; A5YRV1; A6ZID9; B1AVQ8; B1AVR0; B6ECA1; E7ESE5; E7EUG9
• Molecular Weight: 122,102 Da
• NCBI Official Full Name: mucin-1 isoform 3
• NCBI Official Synonym Full Names: mucin 1, cell surface associated
• NCBI Official Symbol: MUC1
• NCBI Official Synonym Symbols: EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MCKD1; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• NCBI Protein Information: mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; tumor-associated mucin; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen
• UniProt Protein Name: Mucin-1
• UniProt Gene Name: MUC1
• UniProt Synonym Gene Names: PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
• UniProt Entry Name: MUC1_HUMAN