Mouse MuRF1 Monoclonal Antibody | anti-MuRF1 antibody

MuRF1 (C-terminal region) M316 Antibody

Cat. Num. AAA71658

• Gene Names: TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Protein G chromatography
• Synonyms: MuRF1, Antibody; MuRF1 (C-terminal region) M316 Antibody; MuRF1 (C-terminal region); MuRF mAb; anti-MuRF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M316
• Specificity: This mouse monoclonal antibody was purified with protein G chromatography. The antibody detects a full length human MuRF1 recombinant protein on SDS-PAGE immunoblots.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-MuRF1 antibody: ELISA, WB (Western Blot)
• Immunogen: Clone M316 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).
• Specificity Note: All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW
• Preparation and Storage: Store at -20°C. Stable for 1 year.

WB (Western Blot)

(Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).)

Related Product Information for anti-MuRF1 antibody

Muscle proteolysis is regulated by the ATP-dependent ubiquitin-proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFalpha treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

References

Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. (2001) Science. 294(5547):1704.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.

NCBI and Uniprot Product Information

• NCBI GI #: 21362898
• NCBI GeneID: 84676
• UniProt Accession #: Q969Q1; Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM63
• NCBI Official Synonym Full Names: tripartite motif containing 63, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM63
• NCBI Official Synonym Symbols: IRF; SMRZ; MURF1; MURF2; RNF28
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM63
• UniProt Gene Name: TRIM63
• UniProt Synonym Gene Names: IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1
• UniProt Entry Name: TRI63_HUMAN