Rabbit MuRF1 Polyclonal Antibody | anti-MuRF1 antibody

MuRF1 (C-terminal region) Antibody

Cat. Num. AAA71659

• Gene Names: TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Synonyms: MuRF1, Antibody; MuRF1 (C-terminal region) Antibody; MuRF1 (C-terminal region); MuRF pAb; anti-MuRF1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using MuRF1 (C-terminal region) peptide (without carrier). The antibody detects 38 and 30kDa* proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38kDa band in mouse heart and muscle tissue.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-MuRF1 antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: MuRF1 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).)

WB (Western Blot)

(Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).)

Related Product Information for anti-MuRF1 antibody

Muscle proteolysis is regulated by the ATP-dependent ubiquitin-proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFalpha treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

References

Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. (2001) Science. 294(5547):1704.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.

NCBI and Uniprot Product Information

• NCBI GI #: 21362898
• NCBI GeneID: 84676
• UniProt Accession #: Q969Q1; Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95
• Molecular Weight: 353
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM63
• NCBI Official Synonym Full Names: tripartite motif containing 63, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM63
• NCBI Official Synonym Symbols: IRF; SMRZ; MURF1; MURF2; RNF28
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM63
• UniProt Gene Name: TRIM63
• UniProt Synonym Gene Names: IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1
• UniProt Entry Name: TRI63_HUMAN