IF (Immunofluorescence)
(ICC/IF analysis of MYD88 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYD88 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human MYD88 Monoclonal Antibody | anti-MYD88 antibody
MYD88 antibody
Gene Names
MYD88; MYD88D
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
MYD88, Antibody; MYD88 antibody; Myeloid differentiation primary response protein MyD88; MYD88D; anti-MYD88 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT22F11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
309
Applicable Applications for anti-MYD88 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human MYD88 (1-309aa) purified from E. coli
Clone Note
Anti-human MYD88 mAb, clone AT22F11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYD88 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of MYD88 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYD88 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of MYD88 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYD88 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates of Raji (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYD88 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The cell lysates of Raji (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYD88 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-MYD88 antibody
Myeloid differentiation primary response gene 88 (MYD88) is adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein increases IL-8 transcription. It is involved in IL-18-mediated signaling pathway. MYD88 activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes.
Product Categories/Family for anti-MYD88 antibody
References
Bonnert TP, et al. (January 1997). FEBS Letters 402 (1): 81-4.; Lord KA, et al. (1990). Oncogene 5 (7): 1095-7.; Arancibia SA, et al. (2007). Biological research 40 (2): 97-112.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,097 Da
NCBI Official Full Name
myeloid differentiation primary response protein MyD88 isoform 2
NCBI Official Synonym Full Names
myeloid differentiation primary response 88
NCBI Official Symbol
MYD88
NCBI Official Synonym Symbols
MYD88D
NCBI Protein Information
myeloid differentiation primary response protein MyD88
UniProt Protein Name
Myeloid differentiation primary response protein MyD88
UniProt Gene Name
MYD88
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Product Notes
The MYD88 myd88 (Catalog #AAA48115) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYD88 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYD88 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MYD88 myd88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYD88, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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