Mouse anti-Human, Mouse Myoglobin Monoclonal Antibody | anti-MB antibody

Mouse anti-human Myoglobin monoclonal antibody

Cat. Num. AAA114272

• Gene Names: MB; PVALB; myoglobgin
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: Myoglobin, Antibody; Mouse anti-human Myoglobin monoclonal antibody; myoglobin;MB;MGC13548;PVALB; anti-MB antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7A3D2
• Specificity: Specific for Human Myoglobin denatured and native forms
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 154

• Applicable Applications for anti-MB antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Myoglobin protein
• Product Type: Monoclonal Antibody
• Immunogen Species: Homo sapiens (Human)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze/thaw.

WB (Western Blot)

(Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissueAll lanes: MB antibody at 1:2000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 18 kDaObserved band size: 18 kDa)

IF (Immunofluorescence)

(Immunofluorescent analysis of MCF-7 cells using AAA114272 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IHC (Immunohistochemisry)

(Immunohistochemical of paraffin-embedded human heart tissue using AAA114272 at dilution of 1:100)

WB (Western Blot)

(Western BlotAll lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1ug/mlLane 1: Myoglobin transfected 293 cell lysateSecondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 18 kDaObserved band size: 20 kDaAdditional bands at: 23, 25, 40 kDa)

WB (Western Blot)

(All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3ug/mlLane 1: Mouse heart muscle lysateLane 2: Recombinant Myoglobin at 10ugSecondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 18 kDaObserved band size: 20 kDaAdditional bands at: 120 kDa)

Related Product Information for anti-MB antibody

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI identification.

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 4885477
• NCBI GeneID: 4151
• NCBI Accession #: NP_005359.1
• NCBI GenBank Nucleotide #: NM_005368.2
• UniProt Accession #: P02144; Q52H51; Q5THY7
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB; myoglobgin
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN