IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedHeLacellswithanti-desmoglein-3antibody5G11(AAA72526) ImmunofluorescenceanalysisofparaformaldehydefixedA431cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofHeLacells(AAA72526)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72526,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
N-cadherin Recombinant Antibody | CDH2 recombinant antibody
Anti-N-cadherin [8C11], Mouse IgG1, kappa
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedHeLacellswithanti-desmoglein-3antibody5G11(AAA72526) ImmunofluorescenceanalysisofparaformaldehydefixedA431cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofHeLacells(AAA72526)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72526,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedHeLacellswithanti-desmoglein-3antibody5G11(AAA72526) ImmunofluorescenceanalysisofparaformaldehydefixedA431cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofHeLacells(AAA72526)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72526,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-N-cadherinantibody8C11(AAA72526). HeLacellswerefixedusing2%PFAandstainedwithanti-unknownspecificityantibodyortherabbitIgG1versionof8C11(AAA72526,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-N-cadherinantibody8C11(AAA72526). HeLacellswerefixedusing2%PFAandstainedwithanti-unknownspecificityantibodyortherabbitIgG1versionof8C11(AAA72526,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer)
WB (Western Blot)
(Western Blotusinganti-N-cadherinantibody8C11(AAA72526) Humanbrain(cerebellum)tissuelysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionof8C11(AAA72526)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-N-cadherinantibody8C11(AAA72526) Humanbrain(cerebellum)tissuelysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionof8C11(AAA72526)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedHeLacellswithanti-desmoglein-3antibody5G11(AAA72526) ImmunofluorescenceanalysisofparaformaldehydefixedA431cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofHeLacells(AAA72526)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72526,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedHeLacellswithanti-desmoglein-3antibody5G11(AAA72526) ImmunofluorescenceanalysisofparaformaldehydefixedA431cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofHeLacells(AAA72526)at10ug/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72526,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Note on publication:
This article describes the generation and characterization of the antibody 8C11.
NCBI and Uniprot Product Information
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Product Notes
The CDH2 cdh2 (Catalog #AAA72526) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-N-cadherin [8C11], Mouse IgG1, kappa reacts with Human, Rabbit, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's N-cadherin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CDH2 cdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-cadherin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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