ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
Rabbit N-Cadherin Polyclonal Antibody | anti-CDH2 antibody
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
N-Cadherin, Antibody; N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Antibody; N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific; Cadherin N 860; anti-CDH2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. In western blots, the antibody detects a 130kDa* band corresponding to N-cadherin (Tyr-860) in human endothelial cells treated with pervanadate and detects a 120kDa band corresponding to E-cadherin (Tyr-835) in human A431 cells treated with pervanadate.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CDH2 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-N-Cadherin (Tyr-860) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 860 in human N-cadherin. This sequence is conserved in human E-cadherin (Tyr-835), P-cadherin (Tyr-782), R-cadherin (Tyr-870), and has significant homology to VE-Cadherin (Tyr-733).
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal N-Cadherin (Cytoplasmic) and rabbit polyclonal N-Cadherin(Tyr-860) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
WB (Western Blot)
(Western blot image of human endothelial cells untreated or treated with pervanadate (1mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).)
WB (Western Blot)
(Western blot image of human endothelial cells untreated or treated with pervanadate (1mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).)
Related Product Information for anti-CDH2 antibody
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
References
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,809 Da
NCBI Official Full Name
cadherin-2 preproprotein
NCBI Official Synonym Full Names
cadherin 2, type 1, N-cadherin (neuronal)
NCBI Official Symbol
CDH2
NCBI Official Synonym Symbols
CDHN; NCAD; CD325; CDw325
NCBI Protein Information
cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
UniProt Protein Name
Cadherin-2
UniProt Gene Name
CDH2
UniProt Synonym Gene Names
CDHN; NCAD; N-cadherin
UniProt Entry Name
CADH2_HUMAN
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Product Notes
The CDH2 cdh2 (Catalog #AAA71602) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's N-Cadherin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDH2 cdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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