WB (Western Blot)
(Western blot analysis of anti-NANOG monoclonal antibody by NANOG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.)
Mouse anti-Human NANOG Monoclonal Antibody | anti-NANOG antibody
NANOG Antibody
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
NANOG, Antibody; NANOG Antibody; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG; anti-NANOG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
60CT77.1.1
Specificity
This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.25 (varies by lot)
Sequence Length
289
Applicable Applications for anti-NANOG antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-NANOG monoclonal antibody by NANOG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.)
WB (Western Blot)
(Western blot analysis of anti-NANOG monoclonal antibody by NANOG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.)
IHC (Immunohiostchemistry)
(NANOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NANOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(NANOG western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).)
WB (Western Blot)
(NANOG western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).)
Related Product Information for anti-NANOG antibody
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Product Categories/Family for anti-NANOG antibody
References
Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010)
Po, A., et al. EMBO J. 29(15):2646-2658(2010)
Zbinden, M., et al. EMBO J. 29(15):2659-2674(2010)
Moretto-Zita, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(30):13312-13317(2010)
Kuijk, E.W., et al. PLoS ONE 5 (6), E10987 (2010) :
Po, A., et al. EMBO J. 29(15):2646-2658(2010)
Zbinden, M., et al. EMBO J. 29(15):2659-2674(2010)
Moretto-Zita, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(30):13312-13317(2010)
Kuijk, E.W., et al. PLoS ONE 5 (6), E10987 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34620
NCBI Official Full Name
homeobox protein NANOG isoform 1
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
NANOG
NCBI Protein Information
homeobox protein NANOG
UniProt Protein Name
Homeobox protein NANOG
UniProt Gene Name
NANOG
UniProt Synonym Gene Names
hNanog
UniProt Entry Name
NANOG_HUMAN
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Product Notes
The NANOG nanog (Catalog #AAA283439) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NANOG Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NANOG can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NANOG nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NANOG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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