Mouse NaVbeta3 Monoclonal Antibody | anti-NaVbeta3 antibody

NaVbeta3 Antibody, Clone S396-29: ATTO 488

Cat. Num. AAA253962

• Gene Names: Scn3b; Scnb3
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: NaVbeta3, Antibody; NaVbeta3 Antibody, Clone S396-29: ATTO 488; Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B; Nav beta3 Antibody: ATTO 488; Sodium channel subunit beta-3 Antibody; Scn3b Antibody; KIAA1158 Antibody; anti-NaVbeta3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: S396-29
• Specificity: Detects ~40 kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% Glycerol, 0.1% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-NaVbeta3 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen Species: Rat
• Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• Conjugate: ATTO 488
• Cellular Localization: Membrane
• Certificate of Analysis: A 1:100 dilution was sufficient for detection of NavBeta3 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Product Notes: Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.; ; Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
• Preparation and Storage: Store at-20 degree C.

WB (Western Blot)

(Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29 at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.)

ICC (Immunocytochemistry)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 3 Monoclonal Antibody, Clone S396-29 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Nav beta 3 Antibody. (D) Composite.)

Related Product Information for anti-NaVbeta3 antibody

Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).

Product Categories/Family for anti-NaVbeta3 antibody

Neuroscience; Ion Channels; Sodium Channels; Voltage-Gated Sodium Channels

References

1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015. 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.

NCBI and Uniprot Product Information

• NCBI GI #: 20806175
• NCBI GeneID: 245956
• NCBI Accession #: NP_620797.1
• NCBI GenBank Nucleotide #: NP_620797.1
• UniProt Accession #: Q9JK00
• NCBI Official Full Name: sodium channel subunit beta-3
• NCBI Official Synonym Full Names: sodium voltage-gated channel beta subunit 3
• NCBI Official Symbol: Scn3b
• NCBI Official Synonym Symbols: Scnb3
• NCBI Protein Information: sodium channel subunit beta-3
• UniProt Protein Name: Sodium channel subunit beta-3
• UniProt Gene Name: Scn3b
• UniProt Entry Name: SCN3B_RAT