IF (Immunofluorescence)
(ICC/IF analysis of NDRG1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NDRG1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human NDRG1 Monoclonal Antibody | anti-NDRG1 antibody
NDRG1 antibody
Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
NDRG1, Antibody; NDRG1 antibody; N-myc downstream regulated 1; CAP43; CMT4D; DRG1; GC4; HMSNL; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated 1 TDD5; 42 kDa; anti-GC4; cmt4d; Differentiation related gene1 protein; Drg 1; hmsnl; Human mRNA for RTP complete cds; NDRG1; tdds; N myc downstream regulated gene 1; N myc downstream regulated gene 1 protein; Ndr 1; ndr1; NDRG 1; NDRG1 protein; Protein nickel specific induction protein; Nickel specific induction protein Cap43; nmsl; Nmyc downstream regulated; reduced in tumor; reducin; Nmyc downstream regulated gene1; Nmyc downstream regulated gene1 protein; Protein regulated by oxygen 1; Protein regulated by oxygen1; proxy1; Reducing agents and tunicamycin responsive protein; tunicamycin-responsive protein; anti-NDRG1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT11G4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
394
Applicable Applications for anti-NDRG1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human NDRG1 (1-394aa) purified from E. coli
Clone Note
Anti-human NDRG1 mAb, clone AT11G4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NDRG1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of NDRG1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NDRG1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of NDRG1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NDRG1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NDRG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : LnCaP cell lysate Lane 2. : HeLa cell lysate Lane 3. : PC3 cell lysate Lane 4. : Hep3B cell lysate Lane 5. : HT1080 cell lysate Lane 6. : Jurkat cell lysate Lane 7. : SW480 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NDRG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : LnCaP cell lysate Lane 2. : HeLa cell lysate Lane 3. : PC3 cell lysate Lane 4. : Hep3B cell lysate Lane 5. : HT1080 cell lysate Lane 6. : Jurkat cell lysate Lane 7. : SW480 cell lysate)
Related Product Information for anti-NDRG1 antibody
N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease.
Product Categories/Family for anti-NDRG1 antibody
References
Van Belzen N., et al. (1997) Lab Invest. 77 (1): 85-92.; Kokame K., et al. (1997) J Biol Chem. 271 (47): 29659-29665. ; Zhang J., et al. (2008) Gene. 417 (1-2): 5-12.; Kachhap SK., et al. (2007) PLoS ONE. 2 (9): e844.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,650 Da
NCBI Official Full Name
protein NDRG1 isoform 1
NCBI Official Synonym Full Names
N-myc downstream regulated 1
NCBI Official Symbol
NDRG1
NCBI Official Synonym Symbols
GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
NCBI Protein Information
protein NDRG1
UniProt Protein Name
Protein NDRG1
UniProt Gene Name
NDRG1
UniProt Synonym Gene Names
CAP43; DRG1; RTP; DRG-1; RTP
Similar Products
Product Notes
The NDRG1 ndrg1 (Catalog #AAA48212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NDRG1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NDRG1 ndrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.