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product-image-AAA286094_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit NDRG1 Polyclonal Antibody | anti-NDRG1 antibody

NDRG1 Antibody (N-term)

Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NDRG1, Antibody; NDRG1 Antibody (N-term); Protein NDRG1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42; NDRG1; CAP43; DRG1; anti-NDRG1 antibody
Ordering
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NDRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human NDRG1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
12-40
Sequence Length
394
Applicable Applications for anti-NDRG1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA286094_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human prostate carcinoma with NDRG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

product-image-AAA286094_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human prostate carcinoma with NDRG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)

product-image-AAA286094_WB15.jpg WB (Western Blot) (Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-NDRG1 antibody
NDRG1 is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis.
Product Categories/Family for anti-NDRG1 antibody
References
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42835
NCBI Official Full Name
protein NDRG1 isoform 1
NCBI Official Synonym Full Names
N-myc downstream regulated 1
NCBI Official Symbol
NDRG1
NCBI Official Synonym Symbols
GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
NCBI Protein Information
protein NDRG1
UniProt Protein Name
Protein NDRG1
UniProt Gene Name
NDRG1
UniProt Synonym Gene Names
CAP43; DRG1; RTP; DRG-1; RTP
UniProt Entry Name
NDRG1_HUMAN

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Product Notes

The NDRG1 ndrg1 (Catalog #AAA286094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 12-40. The NDRG1 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NDRG1 ndrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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