FCM (Flow Cytometry)
(Profile of nephrin expression in human Hek293 cell line analyzed by the BD FACS Canto II. Cells were fixed, permeabilized and stained with anti-Nephrin FITC (green, used 10 ul per test) or with an isotype control (black).)
Rabbit anti-Human Nephrin Monoclonal Antibody | anti-NPHS1 antibody
Anti-Nephrin FITC
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human
Synonyms
Nephrin, Antibody; Anti-Nephrin FITC; Anti-nephrin; anti-NPHS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
D13-E
Form/Format
Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
Sequence Length
1241
Application Notes
Important Notes:
This antibody detects intracellular antigen (cells of interest must be permeabilized).
Optimal dilution should be determined by the end user.
Use 1×106 cells in a 100 ul experimental sample (a test).
Caution: product contains sodium azide.
This antibody detects intracellular antigen (cells of interest must be permeabilized).
Optimal dilution should be determined by the end user.
Use 1×106 cells in a 100 ul experimental sample (a test).
Caution: product contains sodium azide.
Preparation
The antibody was prepared by in vitro cloning technology, and the monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Preparation and Storage
Store undiluted at 4°C, protect from prolonged exposure to light. Do not freeze.
FCM (Flow Cytometry)
(Profile of nephrin expression in human Hek293 cell line analyzed by the BD FACS Canto II. Cells were fixed, permeabilized and stained with anti-Nephrin FITC (green, used 10 ul per test) or with an isotype control (black).)
FCM (Flow Cytometry)
(Profile of nephrin expression in human Hek293 cell line analyzed by the BD FACS Canto II. Cells were fixed, permeabilized and stained with anti-Nephrin FITC (green, used 10 ul per test) or with an isotype control (black).)
Related Product Information for anti-NPHS1 antibody
FITC Rabbit Anti-Human Nephrin
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
NPHS1 nephrin
NCBI Official Symbol
NPHS1
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
NCBI Protein Information
nephrin
UniProt Protein Name
Nephrin
UniProt Gene Name
NPHS1
UniProt Synonym Gene Names
NPHN
UniProt Entry Name
NPHN_HUMAN
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Product Notes
The NPHS1 nphs1 (Catalog #AAA14935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nephrin FITC reacts with Human and may cross-react with other species as described in the data sheet. Important Notes: This antibody detects intracellular antigen (cells of interest must be permeabilized). Optimal dilution should be determined by the end user. Use 1×106 cells in a 100 ul experimental sample (a test). Caution: product contains sodium azide. Researchers should empirically determine the suitability of the NPHS1 nphs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nephrin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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