Standard Curve (Sample)
Rat Nephrin ELISA Kit | Nephrin elisa kit
Rat Nephrin ELISA Kit
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Rat
Synonyms
Nephrin; N/A; Rat Nephrin ELISA Kit; Nephrin elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of NPHN. No significant cross-reactivity or interference between NPHN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPHN and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Nephrin elisa kit
Intended Uses: This NPHN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NPHN. This ELISA kit for research use only!
Principle of the Assay: NPHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NPHN antibody and an NPHN-HRP conjugate. The assay sample and buffer are incubated together with NPHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPHN concentration since NPHN from samples and NPHN-HRP conjugate compete for the anti-NPHN antibody binding site. Since the number of sites is limited, as more sites are occupied by NPHN from the sample, fewer sites are left to bind NPHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPHN concentration in each sample is interpolated from this standard curve.
Principle of the Assay: NPHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NPHN antibody and an NPHN-HRP conjugate. The assay sample and buffer are incubated together with NPHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPHN concentration since NPHN from samples and NPHN-HRP conjugate compete for the anti-NPHN antibody binding site. Since the number of sites is limited, as more sites are occupied by NPHN from the sample, fewer sites are left to bind NPHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPHN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
130,546 Da
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol
NPHS1
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
NCBI Protein Information
nephrin; renal glomerulus-specific cell adhesion receptor
UniProt Protein Name
Nephrin
UniProt Gene Name
NPHS1
UniProt Synonym Gene Names
NPHN
UniProt Entry Name
NPHN_HUMAN
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Product Notes
The Rat Nephrin nphs1 (Catalog #AAA16159) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16159 ELISA Kit recognizes Rat Nephrin. It is sometimes possible for the material contained within the vial of "Nephrin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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