ICC (Immunocytochemistry)
(1. Coat coverslips with 1% gelatin-coating solution for 2 hours at room temperature (RT); rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS. 2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT. 3. Wash 2 x 3 min with PBS. 4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS, pH 7.2) for 5 min on ice. 5. Wash 2 x 3 min with PBS. 6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT. 7. Incubate the cells with primary antibody: anti-Nephrin clonal antibody at the dilution of 1:600 - 1:1000 in antibody dilution buffer (PBS, 1% BSA) for 1 hour at RT in humid chamber. 8. Wash 2 x 3 min with PBS. 9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, was used at 1:300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark). 10. Wash 3 x 3 min with PBS. 11. Rinse once with distilled water. 12. Mount the slide for observation, with a drop of anti-fade mounting medium. )
Rabbit NEPHRIN Monoclonal Antibody | anti-NEPHRIN antibody
Anti - NEPHRIN
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human, Mouse, Rat - tested
Applications
ELISA, Western Blot, Immunoprecipitation
Synonyms
NEPHRIN, Antibody; Anti - NEPHRIN; Rabbit anti-nephrin clonal IgGs; anti-NEPHRIN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat - tested
Clonality
Monoclonal
Clone Number
Y17-R
Form/Format
20 mM Tris-HCl, pH 8.0
Applicable Applications for anti-NEPHRIN antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ELISA - 1:50,000 - 1:100,000
WB - 1:5,000
WB - 1:5,000
Stabilizer
10 mg/ml BSA
Preservative
0.05% Sodium Azide
Clone Note
Major clone of rabbit immunoglobulin corresponding to immunogenic peptide
Immunogen
Peptide derived from the center of the fifth Ig-like domain of human nephrin.
Antibody recognizes the epitope located between Cys465 - Cys528.
Antibody recognizes the epitope located between Cys465 - Cys528.
Precautions
1.Intended for professional in Vitro test use in laboratories
2.Do not use after expiration date printed on label
3.Avoid contamination of the reagent
4.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results
5.The reagent contains sodium azide(NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
6.Disposal of waste material must be conducted in accordance with local regulations
7. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2.Do not use after expiration date printed on label
3.Avoid contamination of the reagent
4.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results
5.The reagent contains sodium azide(NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
6.Disposal of waste material must be conducted in accordance with local regulations
7. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
10 ul aliquots at -20°C
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(1. Coat coverslips with 1% gelatin-coating solution for 2 hours at room temperature (RT); rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS. 2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT. 3. Wash 2 x 3 min with PBS. 4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS, pH 7.2) for 5 min on ice. 5. Wash 2 x 3 min with PBS. 6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT. 7. Incubate the cells with primary antibody: anti-Nephrin clonal antibody at the dilution of 1:600 - 1:1000 in antibody dilution buffer (PBS, 1% BSA) for 1 hour at RT in humid chamber. 8. Wash 2 x 3 min with PBS. 9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, was used at 1:300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark). 10. Wash 3 x 3 min with PBS. 11. Rinse once with distilled water. 12. Mount the slide for observation, with a drop of anti-fade mounting medium. )
ICC (Immunocytochemistry)
(1. Coat coverslips with 1% gelatin-coating solution for 2 hours at room temperature (RT); rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS. 2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT. 3. Wash 2 x 3 min with PBS. 4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS, pH 7.2) for 5 min on ice. 5. Wash 2 x 3 min with PBS. 6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT. 7. Incubate the cells with primary antibody: anti-Nephrin clonal antibody at the dilution of 1:600 - 1:1000 in antibody dilution buffer (PBS, 1% BSA) for 1 hour at RT in humid chamber. 8. Wash 2 x 3 min with PBS. 9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, was used at 1:300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark). 10. Wash 3 x 3 min with PBS. 11. Rinse once with distilled water. 12. Mount the slide for observation, with a drop of anti-fade mounting medium. )
WB (Western Blot)
(Western immunoblotting solutions:- Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100- Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milkFor western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)
WB (Western Blot)
(Western immunoblotting solutions:- Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100- Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milkFor western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)
Related Product Information for anti-NEPHRIN antibody
Rabbit anti-nephrin clonal IgGS
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Nephrin
NCBI Official Synonym Full Names
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol
NPHS1
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
NCBI Protein Information
nephrin; renal glomerulus-specific cell adhesion receptor
UniProt Protein Name
Nephrin
UniProt Gene Name
NPHS1
UniProt Synonym Gene Names
NPHN
UniProt Entry Name
NPHN_HUMAN
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Product Notes
The NEPHRIN nphs1 (Catalog #AAA14932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - NEPHRIN reacts with Human, Mouse, Rat - tested and may cross-react with other species as described in the data sheet. AAA Biotech's NEPHRIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). ELISA - 1:50,000 - 1:100,000 WB - 1:5,000. Researchers should empirically determine the suitability of the NEPHRIN nphs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEPHRIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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