Mouse NF-kB p65/RELA Monoclonal Antibody | anti-RELA antibody

NF-kB p65/RELA Mouse Monoclonal Antibody [Clone PCRP-RELA-1E3]

Cat. Num. AAA215890

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human; Predicted to react with Rat.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: NF-kB p65/RELA, Antibody; NF-kB p65/RELA Mouse Monoclonal Antibody [Clone PCRP-RELA-1E3]; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Transcription factor p65; V rel avian reticuloendotheliosis viral oncogene homolog A; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65; NFKB3; anti-RELA antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-RELA-1E3
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-RELA antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human RELA protein
• Cellular Localization: Nucleus. Also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B), Colocalized with RELA in the nucleus upon TNF-alpha induction
• Positive Control: HeLa, U87, K562 or MCF-7 cells.
• Hu-chromosome Location: 11q13.1
• Unigene: 502875
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed K562 cells. NF-kB p65/RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)

Related Product Information for anti-RELA antibody

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 60,219 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN