Mouse anti-Human NFKB1 Monoclonal Antibody | anti-NFKB1 antibody

NFKB1 Antibody

Cat. Num. AAA283497

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: NFKB1, Antibody; NFKB1 Antibody; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p50 subunit; NFKB1; anti-NFKB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Clone Number: 1298CT792.105.117.133
• Specificity: This antibody is generated from a mouse immunized with a recombinant protein from human NFKB1.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 968

• Applicable Applications for anti-NFKB1 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Antigen Type: Recombinant Protein
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from Daudi, Jurkat cell line (from left to right), using NFKB1 Antibody. AAA283497 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded H. tonsil section using NFKB1. AAA283497 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded H. spleen section using NFKB1. AAA283497 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Hela cells using NFKB1(green) compared to an isotype control of mouse IgG1(blue). AAA283497 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

Related Product Information for anti-NFKB1 antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Product Categories/Family for anti-NFKB1 antibody

Crown Antibodies; Cancer; Cell Biology; Metabolism

References

Kieran M.,et al.Cell 62:1007-1018(1990).
Bours V.,et al.Nature 348:76-80(1990).
Meyer R.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:966-970(1991).
Heron E.,et al.Genomics 30:493-505(1995).
Chang H.-M.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 259155302
• NCBI GeneID: 4790
• NCBI Accession #: NP_001158884.1
• NCBI GenBank Nucleotide #: NM_001165412.1
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 105356
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 2
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN