Rabbit NGF-Receptor (p75)/CD271 Monoclonal Antibody | anti-NGFR antibody

NGF-Receptor (p75)/CD271 (Soft Tissue Tumor Marker)

Cat. Num. AAA214465

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human, Non-human Primates.; Does not react with Mouse, Rat. Others not known.
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: NGF-Receptor (p75)/CD271, Antibody; NGF-Receptor (p75)/CD271 (Soft Tissue Tumor Marker); CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor (NGFR); p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16); anti-NGFR antibody

• Host: Rabbit
• Reactivity: Human, Non-human Primates.; Does not react with Mouse, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: NGFR/1997R
• Specificity: It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 427

• Applicable Applications for anti-NGFR antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human p75 NGFR protein
• Cellular Localization: Cell Surface and Cytoplasmic
• Hu-chromosome Location: 17q21-22
• Positive Control: Neuronal axons, Schwann cells, and perineural cells of peripheral nerves, or tumors of nerve sheath differentiation e.g. Schwannoma, Neurofibroma. Soma and axons of sensory neurons, and ganglionic satellite cells. Melanomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R).)

References

Wang, W., et al. 2009. Patterns of expression and function of the p75NGFR protein in pancreatic cancer cells and tumours. Eur. J. Surg. Oncol. 35: 826-832. Soland, T.M., et al. 2008. Nerve growth factor receptor (p75NTR) and pattern of invasion predict poor prognosis in oral squamous cell carcinoma. Histopathology 53: 62-72.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961; B4E096
• Molecular Weight: 75kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor