NKG2D recombinant antibody

Anti-NKG2D [CX-5], Rat IgG1, lambda

Cat. Num. AAA72535

• Gene Names: Klrk1; Nkg2d; NKG2-D; D6H12S2489E
• Reactivity: Mouse
• Applications: Neutralization Assay, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: NKG2D, Recombinant Antibody; Anti-NKG2D [CX-5], Rat IgG1, lambda; NKG2D; Anti-NKG2D [CX-5]; CD314; KLRK1; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CX5; NKG2D recombinant antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 lambda
• Clone Number: CX-5
• Specificity: This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for NKG2D recombinant antibody: NTRL (Neutralization Assay), Blocking, FCM/FACS (Flow Cytometry)
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(FlowcytometryusingtheAnti-NKG2DantibodyCX-5(AAA72535). Paraformaldehydefixedmousesplenocyteswerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofCX-5(AAA72535,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)

IF (Immunofluorescence)

(ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2D(AAA72535)CX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(AAA72535)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72535,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)

WB (Western Blot)

(Western BlotusingAnti-NKG2DantibodyCX-5(AAA72535). Mouselymphnodetissuelysate(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofCX-5(AAA72535)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)

FCM/FACS (Flow Cytometry)

(FlowcytometryusingtheAnti-NKG2DantibodyCX-5(AAA72535). Paraformaldehydefixedmousesplenocyteswerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofCX-5(AAA72535,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)

Related Product Information for NKG2D recombinant antibody

The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1.

References

Ogasawara et al. Impairment of NK cell function by NKG2D modulation in NOD mice Immunity. 2003 Jan;18(1):41-51. doi: 10.1016/s1074-7613(02)00505-8. N/A12530974
Note on publication:
Describes the characterization of this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 134023658
• NCBI GeneID: 27007
• NCBI Accession #: NP_001076791.1
• NCBI GenBank Nucleotide #: NM_001083322.1
• UniProt Accession #: O54709
• Molecular Weight: 26,710 Da
• NCBI Official Full Name: NKG2-D type II integral membrane protein isoform b
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily K, member 1
• NCBI Official Symbol: Klrk1
• NCBI Official Synonym Symbols: Nkg2d; NKG2-D; D6H12S2489E
• NCBI Protein Information: NKG2-D type II integral membrane protein; NK cell receptor D; natural killer cell group 2D; NKG2-D-activating NK receptor
• UniProt Protein Name: NKG2-D type II integral membrane protein
• UniProt Gene Name: Klrk1
• UniProt Synonym Gene Names: Nkg2d
• UniProt Entry Name: NKG2D_MOUSE