IF (Immunofluorescence)
(Immunofluorescence staining of human peripheral blood leukocytes with anti-CD314 (AAA72273) 6E5A7 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes stained with the chimeric rabbit IgG version of 6E5A7 (AAA72273-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72273-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human NKG2D Antibody | anti-NKG2D antibody
Anti-NKG2D [6E5A7], Mouse IgG2b
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
NKG2D; N/A; Anti-NKG2D [6E5A7], Mouse IgG2b; Anti-NKG2D [6E5A7]; CD314; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; KLRK1; anti-NKG2D antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2b, Kappa
Clone Number
6E5A7
Specificity
This antibody recognizes and binds the extracellular epitope of NKG2D and blocks the interaction between ligand and receptor.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NKG2D antibody
Blocking, FCM/FACS (Flow Cytometry)
Immunogen
The original antibody was generated by immunizing BALB/c mice with soluble NKG2-D fragments containing amino acids from 64-462 and 123-462.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of human peripheral blood leukocytes with anti-CD314 (AAA72273) 6E5A7 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes stained with the chimeric rabbit IgG version of 6E5A7 (AAA72273-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72273-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of human peripheral blood leukocytes with anti-CD314 (AAA72273) 6E5A7 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes stained with the chimeric rabbit IgG version of 6E5A7 (AAA72273-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72273-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CD314 antibody 6E5A7 (AAA72273). K562(A) (2ug/ml) and human peripheral blood monocytes(B) (2ug/ml) cells lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 6E5A7 (AAA72273-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD314 antibody 6E5A7 (AAA72273). K562(A) (2ug/ml) and human peripheral blood monocytes(B) (2ug/ml) cells lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 6E5A7 (AAA72273-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-NKG2D antibody
The NKG2D (Natural Killer Group 2 Member D) protein is an activating receptor that enables NK and CD8 + T cells to recognize and eliminate infected or transformed endogenous cells. However, an incorrect regulation of this receptor on immune cells also appears to lead to the development of autoimmune diseases such as type I diabetes, celiac disease and RA.This antibody positively reacts with NKG2D on CD8-positive T cells and to CD56-positive natural killer cells. The binding was confirmed FACS analysis (Steigerwald, 2008).
Product Categories/Family for anti-NKG2D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,274 Da
NCBI Official Full Name
NKG2-D type II integral membrane protein
NCBI Official Symbol
KLRC4-KLRK1
NCBI Protein Information
NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor; killer cell lectin-like receptor subfamily K member 1
UniProt Protein Name
NKG2-D type II integral membrane protein
UniProt Gene Name
KLRK1
UniProt Synonym Gene Names
D12S2489E; NKG2D
UniProt Entry Name
NKG2D_HUMAN
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Product Notes
The NKG2D klrk1 (Catalog #AAA72273) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NKG2D [6E5A7], Mouse IgG2b reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NKG2D can be used in a range of immunoassay formats including, but not limited to, Blocking, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NKG2D klrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKG2D, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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