Mouse nNOS Monoclonal Antibody | anti-nNOS antibody

nNOS (C-terminal region) M401 Antibody

Cat. Num. AAA71673

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Human, Rat, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot
• Purity: Protein A chromatography
• Synonyms: nNOS, Antibody; nNOS (C-terminal region) M401 Antibody; nNOS (C-terminal region); NOSn m; anti-nNOS antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: M401
• Specificity: The antibody detects a 155kDa* protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates, as well in the rat pituitary cell line GC.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-nNOS antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone (M401) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human nNOS. This human nNOS sequence has high homology with similar regions in rat and mouse nNOS.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.)

WB (Western Blot)

(Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).)

Related Product Information for anti-nNOS antibody

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.

References

Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870.
Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343.
Xie, Q.W. et al. (1992) Science 256:225.

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 160,970 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN