Rabbit nNOS Polyclonal Antibody | anti-nNOS antibody

Phospho-nNOS (Ser1417) Antibody

Cat. Num. AAA329778

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: nNOS, Antibody; Phospho-nNOS (Ser1417) Antibody; 2310005C01Rik; BNOS; Constitutive NOS; EC 1.14.13.39; IHPS 1; IHPS1; N-NOS; NC-NOS; neuronal Nitric Oxide Synthase; Neuronal NOS; Nitric oxide synthase, neuronal, included; Nitric oxide synthase 1 (neuronal); Nitric oxide synthase 1; Nitric oxide synthase, brain; Nitric oxide synthase, penile neuronal, included; NNOS; NO; NOS 1; NOS; NOS type I; NOS-I; NOS1; NOS1_HUMAN; Peptidyl-cysteine S-nitrosylase NOS1; anti-nNOS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-nNOS (Ser1417) Antibody detects endogenous levels of nNOS only when phosphorylated at Ser1417.; Tissue Specificity: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-nNOS antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human nNOS around the phosphorylation site of Ser1417.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
• Subunit Structure: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4. Interacts with VAC14 (By similarity). Interacts with SLC6A4 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).
• Similarity: The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity).Belongs to the NOS family.
• Subcellular Location: Cell membrane>Sarcolemma>Peripheral membrane protein. Cell projection>Dendritic spine.; Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(Staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis nNOS (Phospho-Ser1417) using HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-nNOS antibody

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 160,970 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN