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IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
Rabbit anti-Human NRF2 Monoclonal Antibody | anti-NRF2 antibody
NRF2 (Phospho-S40) Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
NRF2, Antibody; NRF2 (Phospho-S40) Antibody; erythroid derived 2 antibody; HEBP1 antibody; like 2 antibody; NF E2 related factor 2 antibody; NF-E2-related factor 2 antibody; NF2L2_HUMAN antibody; NFE2 related factor 2 antibody; NFE2-related factor 2 antibody; Nfe2l2 antibody; Nrf 2 antibody; NRF2 antibody; Nuclear factor (erythroid derived 2) like 2 antibody; Nuclear factor antibody; nuclear factor erythroid 2 like 2 antibody; Nuclear factor erythroid 2 related factor 2 antibody; Nuclear factor erythroid 2-related factor 2 antibody; Nuclear factor erythroid derived 2 like 2 antibody; anti-NRF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SU0334
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for anti-NRF2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
IP: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
IP: 1:50
Antibody Type
Recombinant Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser40 of human Nrf2.
Preparation and Storage
Store at -20°C.
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
WB (Western Blot)
(Erjingwan Extracts Exert Antiaging Effects of Skin through Activating Nrf2 and Inhibiting NF-B. -Evidence-based Complementary and Alternative Medicine)
WB (Western Blot)
(Erjingwan Extracts Exert Antiaging Effects of Skin through Activating Nrf2 and Inhibiting NF-B. -Evidence-based Complementary and Alternative Medicine)
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Nrf2 (S40) Antibody.)
WB (Western Blot)
(All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.)
WB (Western Blot)
(All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Phospho-Nrf2 (S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Phospho-Nrf2 (S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-Nrf2 (S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Phospho-Nrf2 (S40) on different lysates using anti-Phospho-Nrf2 (S40) antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Raji)
WB (Western Blot)
(Western blot analysis of Phospho-Nrf2 (S40) on different lysates using anti-Phospho-Nrf2 (S40) antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Raji)
Related Product Information for anti-NRF2 antibody
The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap n collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1. The gene encoding human Nrf3 maps to chromosome 7p15.2. Nrf3 is highly expressed in placenta, B cells and monocytes.
Product Categories/Family for anti-NRF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 90 kDa
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor (erythroid-derived 2)-like 2
NCBI Official Symbol
NFE2L2
NCBI Official Synonym Symbols
NRF2
NCBI Protein Information
nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2
UniProt Entry Name
NF2L2_HUMAN
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Product Notes
The NRF2 nfe2l2 (Catalog #AAA29775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NRF2 (Phospho-S40) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NRF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 FC/FACS: 1:50 IP: 1:50. Researchers should empirically determine the suitability of the NRF2 nfe2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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