Porcine Nuclear factor erythroid 2-related factor 2 ELISA Kit | Nrf2 elisa kit

Porcine Nuclear factor erythroid 2-related factor 2 ELISA Kit

Cat. Num. AAA96395

• Gene Names: NFE2L2; NRF2
• Reactivity: Porcine
• Synonyms: Nuclear factor erythroid 2-related factor 2; N/A; Porcine Nuclear factor erythroid 2-related factor 2 ELISA Kit; Nrf2 elisa kit

• Reactivity: Porcine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive

• Intended Uses: This Nrf2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Nrf2. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Nrf2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Nrf2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Nrf2 elisa kit

Principle of the Assay: Nrf2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Nrf2 antibody and an Nrf2-HRP conjugate. The assay sample and buffer are incubated together with Nrf2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Nrf2 concentration since Nrf2 from samples and Nrf2-HRP conjugate compete for the anti-Nrf2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Nrf2 from the sample, fewer sites are left to bind Nrf2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Nrf2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224028257
• NCBI GeneID: 4780
• NCBI Accession #: NP_001138884.1
• NCBI GenBank Nucleotide #: NM_001145412.2
• Molecular Weight: 65,356 Da
• NCBI Official Full Name: nuclear factor erythroid 2-related factor 2 isoform 2
• NCBI Official Synonym Full Names: nuclear factor, erythroid 2-like 2
• NCBI Official Symbol: NFE2L2
• NCBI Official Synonym Symbols: NRF2
• NCBI Protein Information: nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2
• UniProt Protein Name: Nuclear factor erythroid 2-related factor 2
• UniProt Gene Name: NFE2L2
• UniProt Synonym Gene Names: NRF2; NF-E2-related factor 2; NFE2-related factor 2
• UniProt Entry Name: NF2L2_HUMAN