Standard Curve (Sample)
Chicken Nuclear factor erythroid 2-related factor 2 ELISA Kit | Nrf2 elisa kit
Chicken Nuclear factor erythroid 2-related factor 2 ELISA Kit
Gene Names
NFE2L2; NRF2
Reactivity
Chicken
Synonyms
Nuclear factor erythroid 2-related factor 2; Chicken Nuclear factor erythroid 2-related factor 2 ELISA Kit; Nrf2 elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Nrf2 elisa kit
Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.
Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for Nrf2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,356 Da
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor, erythroid 2-like 2
NCBI Official Symbol
NFE2L2
NCBI Official Synonym Symbols
NRF2
NCBI Protein Information
nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2; NFE2-related factor 2
UniProt Entry Name
NF2L2_HUMAN
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Product Notes
The Chicken Nrf2 nfe2l2 (Catalog #AAA17229) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17229 ELISA Kit recognizes Chicken Nrf2. It is sometimes possible for the material contained within the vial of "Nuclear factor erythroid 2-related factor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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