FCM/FACS (Flow Cytometry)
(Flow Cytometry of Human Nuclear Antigen on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).)
Mouse Nuclear Antigen (HNA) Monoclonal Antibody
Human Nuclear Antigen (HNA) (Human Cell Marker) Mouse Monoclonal Antibody
Reactivity
Human, Non-Human Primates.Does not react with Mouse, Rat and Chicken
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab conjugated to PE
Synonyms
Nuclear Antigen (HNA), Antibody; Human Nuclear Antigen (HNA) (Human Cell Marker) Mouse Monoclonal Antibody; HNA; HuNu; anti-Nuclear Antigen (HNA) antibody
Host
Mouse
Reactivity
Human, Non-Human Primates.
Does not react with Mouse, Rat and Chicken
Does not react with Mouse, Rat and Chicken
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
235-1
Specificity
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Purity/Purification
Purified Ab conjugated to PE
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Applicable Applications for anti-Nuclear Antigen (HNA) antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Nuclei
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Positive Control
All human cells or tissues
Preparation and Storage
Antibody with azide - store at 4 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
FCM/FACS (Flow Cytometry)
(Flow Cytometry of Human Nuclear Antigen on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).)
FCM/FACS (Flow Cytometry)
(Flow Cytometry of Human Nuclear Antigen on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).)
FCM/FACS (Flow Cytometry)
(Flow Cytometry of Human Nuclear Antigen on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).)
FCM/FACS (Flow Cytometry)
(Flow Cytometry of Human Nuclear Antigen on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).)
References
Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseas
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Product Notes
The Nuclear Antigen (HNA) (Catalog #AAA62719) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human Nuclear Antigen (HNA) (Human Cell Marker) Mouse Monoclonal Antibody reacts with Human, Non-Human Primates. Does not react with Mouse, Rat and Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Antigen (HNA) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Nuclear Antigen (HNA) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Antigen (HNA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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