IF (Immunofluorescence)
(ICC/IF analysis of NUDT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NUDT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human NUDT1 Monoclonal Antibody | anti-NUDT1 antibody
NUDT1 antibody
Gene Names
NUDT1; MTH1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
NUDT1, Antibody; NUDT1 antibody; 78-dihydro-8-oxoguanine triphosphatase; MTH1; 8-oxo-dGTPase; Nudix motif 1; 7,8-dihydro-8-oxoguanine triphosphatase; anti-NUDT1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT3B3
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
156
Applicable Applications for anti-NUDT1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human NUDT1 (1-156aa) purified from E. coli
Clone Note
Anti-human NUDT1 mAb, clone AT3B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NUDT1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of NUDT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NUDT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of NUDT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NUDT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NUDT1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K-562 cell lysate Lane 2.: SW480 cell lysate Lane 3.: THP-1 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: A549 cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NUDT1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K-562 cell lysate Lane 2.: SW480 cell lysate Lane 3.: THP-1 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: A549 cell lysate)
Related Product Information for anti-NUDT1 antibody
NUDT1, also known as MTH1 is a 179 amino acid cytoplasmic protein that is a member of the nudix hydrolase family. Highly expressed in testis, thymus and proliferating blood lymphocytes, NUDT1 functions as an antimutagenic that hydrolyzes oxidized purine nucleoside triphosphates to their corresponding monophosphates. Through its ability to enzymatically hydrolyze ATP and GTP to AMP and GMP, respectively, NUDT1 prevents misincorporation of GTP into DNA, thus preventing A:T to C:G transversions.
Product Categories/Family for anti-NUDT1 antibody
References
Satoh J., et al. (2000) Eur J Neurol. 7 (6): 673-677. ; Choi KM., et al. (2015) FEBS Lett. 589 (3): 349-357.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,952 Da
NCBI Official Full Name
7,8-dihydro-8-oxoguanine triphosphatase isoform p18
NCBI Official Synonym Full Names
nudix hydrolase 1
NCBI Official Symbol
NUDT1
NCBI Official Synonym Symbols
MTH1
NCBI Protein Information
7,8-dihydro-8-oxoguanine triphosphatase
UniProt Protein Name
7,8-dihydro-8-oxoguanine triphosphatase
UniProt Gene Name
NUDT1
UniProt Synonym Gene Names
MTH1; Nudix motif 1
Similar Products
Product Notes
The NUDT1 nudt1 (Catalog #AAA48185) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NUDT1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NUDT1 nudt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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