IHC (Immunohiostchemistry)
(Immunohistochemical analysis of NUP50 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
NUP50 recombinant antibody
Recombinant Anti-NUP50 Rabbit mAb
Gene Names
NUP50; NPAP60; NPAP60L
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
NUP50, Recombinant Antibody; Recombinant Anti-NUP50 Rabbit mAb; NPAP60L; Nuclear pore complex protein Nup50; 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50; NUP50 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of NUP50 protein
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS, pH7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Applicable Applications for NUP50 recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human NUP50. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of NUP50 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of NUP50 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
WB (Western Blot)
(Western blot analysis of NUP50 expression in HEK293T (A), Jurkat (B), THP1 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD))
WB (Western Blot)
(Western blot analysis of NUP50 expression in HEK293T (A), Jurkat (B), THP1 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD))
Related Product Information for NUP50 recombinant antibody
Recombinant rabbit monoclonal antibody to NUP50
Product Categories/Family for NUP50 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,144 Da
NCBI Official Full Name
nuclear pore complex protein Nup50 isoform b
NCBI Official Synonym Full Names
nucleoporin 50kDa
NCBI Official Symbol
NUP50
NCBI Official Synonym Symbols
NPAP60; NPAP60L
NCBI Protein Information
nuclear pore complex protein Nup50; nucleoporin 50kD; nucleoporin Nup50; 50 kDa nucleoporin; nuclear pore-associated protein 60L
UniProt Protein Name
Nuclear pore complex protein Nup50
UniProt Gene Name
NUP50
UniProt Synonym Gene Names
NPAP60L
UniProt Entry Name
NUP50_HUMAN
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Product Notes
The NUP50 nup50 (Catalog #AAA266340) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-NUP50 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUP50 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NUP50 nup50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP50, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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