WB (Western Blot)
(Detection of human NUP50 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NUP50 antibody AAA211630 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human NUP50 Polyclonal Antibody | anti-NUP50 antibody
Rabbit anti-NUP50 Antibody, Affinity Purified
Gene Names
NUP50; NPAP60; NPAP60L
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NUP50, Antibody; Rabbit anti-NUP50 Antibody, Affinity Purified; nucleoporin Nup50; 50 kDa nucleoporin; NPAP60; NPAP60L; nuclear pore complex protein Nup50; Nuclear pore-associated protein 60 kDa-like; nuclear pore-associated protein 60L; nucleoporin 50kD; nucleoporin 50kDa; Nuclear pore complex protein Nup50; nucleoporin 50; anti-NUP50 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NUP50 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NUP50 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NUP50 antibody AAA211630 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human NUP50 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NUP50 antibody AAA211630 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human NUP50 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NUP50 (Cat. No. AAA211630 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NUP50 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NUP50 (Cat. No. AAA211630 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NUP50 antibody
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Nup50 (also called Npap60) appears to not be a fixed structural component of the NPC. Nup50 has been shown to be a component of the importin complex, a protein carrier complex that imports cargo from the cytoplasm to the nucleus through the NPC. In this complex, Nup50 appears to have importance in the late stages of import and functions in complex disassembly and importin recycling.
Product Categories/Family for anti-NUP50 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,144 Da
NCBI Official Full Name
nuclear pore complex protein Nup50 isoform b
NCBI Official Synonym Full Names
nucleoporin 50kDa
NCBI Official Symbol
NUP50
NCBI Official Synonym Symbols
NPAP60; NPAP60L
NCBI Protein Information
nuclear pore complex protein Nup50; nucleoporin 50kD; nucleoporin Nup50; 50 kDa nucleoporin; nuclear pore-associated protein 60L
UniProt Protein Name
Nuclear pore complex protein Nup50
UniProt Gene Name
NUP50
UniProt Synonym Gene Names
NPAP60L
UniProt Entry Name
NUP50_HUMAN
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Product Notes
The NUP50 nup50 (Catalog #AAA211630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NUP50 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUP50 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUP50 nup50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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