Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using OLIG2 Mouse Monoclonal Antibody (OLIG2/2400) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse OLIG2 Monoclonal Antibody | anti-OLIG2 antibody
OLIG2 (Marker of Glial Brain Tumors)
Gene Names
OLIG2; BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
OLIG2, Antibody; OLIG2 (Marker of Glial Brain Tumors); Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); OLIG2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; Protein kinase C-binding protein 2 (PRKCBP2); RACK17; anti-OLIG2 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
OLIG2/2400
Specificity
Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
323
Applicable Applications for anti-OLIG2 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant fragment of human OLIG2 protein (around aa 1-141)
Cellular Localization
Nuclear
Hu-chromosome Location
21q22.11
Positive Control
THP-1 cells. Astrocytoma
Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using OLIG2 Mouse Monoclonal Antibody (OLIG2/2400) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using OLIG2 Mouse Monoclonal Antibody (OLIG2/2400) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified OLIG2 Mouse Monoclonal Antibody (OLIG2/2400).Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified OLIG2 Mouse Monoclonal Antibody (OLIG2/2400).Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with OLIG2 Mouse Monoclonal Antibody (OLIG2/2400).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with OLIG2 Mouse Monoclonal Antibody (OLIG2/2400).)
References
Mokhtari K, et al. Olig2 expression, GFAP, p53 and 1p loss analysis contribute to glioma subclassification. Neuropathol Appl Neurobiol. 2005; 31:62-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30-40kDa
NCBI Official Full Name
oligodendrocyte transcription factor 2
NCBI Official Synonym Full Names
oligodendrocyte transcription factor 2
NCBI Official Symbol
OLIG2
NCBI Official Synonym Symbols
BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
NCBI Protein Information
oligodendrocyte transcription factor 2
UniProt Protein Name
Oligodendrocyte transcription factor 2
UniProt Gene Name
OLIG2
UniProt Synonym Gene Names
BHLHB1; BHLHE19; PRKCBP2; RACK17; Oligo2; bHLHb1; bHLHe19
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Product Notes
The OLIG2 olig2 (Catalog #AAA214640) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The OLIG2 (Marker of Glial Brain Tumors) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's OLIG2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the OLIG2 olig2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OLIG2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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